bs-14262R [Primary Antibody]
delta 1 Catenin (Tyr280) Polyclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
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DATASHEET

Host: Rabbit

Target Protein: delta 1 Catenin (Tyr280)

Modification Site: Tyr280

Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 1500

Swiss Prot: O60716

Source: KLH conjugated synthesised phosphopeptide derived from human delta 1 Catenin around the phosphorylation site of Tyr280

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Background:

This gene encodes a member of the Armadillo protein family, which function in adhesion between cells and signal transduction. Multiple translation initiation codons and alternative splicing result in many different isoforms being translated. Not all of the full-length natures of the described transcript variants have been determined. Read-through transcription also exists between this gene and the neighboring upstream thioredoxin-related transmembrane protein 2 (TMX2) gene. [provided by RefSeq, Dec 2010]

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)
ELISA(1:500-1000)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)
ICC(1:100-500)

Predicted Molecular Weight: 106


Cross Reactive Species: Mouse

Predicted Cross Reactive Species: Human
Rat
Dog
Cow
Pig
Horse

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Paraformaldehyde-fixed, paraffin embedded Mouse brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with delta 1 Catenin (Tyr280) Polyclonal Antibody, Unconjugated (bs-14262R) at 1:400 overnight at 4°C, DAB staining.