bs-14298R [Primary Antibody]
DGKH Polyclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: DGKH

Immunogen Range: 51-150/1220


Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 160851

Swiss Prot: Q86XP1

Source: KLH conjugated synthetic peptide derived from human DGKH

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.

Storage: Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.

Background:

Phosphorylates diacylglycerol (DAG) to generate phosphatidic acid (PA). Plays a key role in promoting cell growth. Activates the Ras/B-Raf/C-Raf/MEK/ERK signaling pathway induced by EGF. Regulates the recruitment of RAF1 and BRAF from cytoplasm to membranes and their heterodimerization.

Size: 100ul

Concentration: 1ug/ul

Applications: ELISA(1:500-1000)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)
ICC(1:100-500)

Predicted Molecular Weight: 135


Cross Reactive Species: Human
Rat

Predicted Cross Reactive Species: Mouse
Pig
Chicken
Rabbit

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Paraformaldehyde-fixed, paraffin embedded rat kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with DGKH Polyclonal Antibody, Unconjugated (bs-14298R) at 1:400 overnight at 4°C, followed by a conjugated secondary for 20 minutes and DAB staining.


Paraformaldehyde-fixed, paraffin embedded rat heart; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with DGKH Polyclonal Antibody, Unconjugated (bs-14298R) at 1:400 overnight at 4°C, followed by a conjugated secondary for 20 minutes and DAB staining.