bs-1434R [Primary Antibody]
IGFBP3 Polyclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: IGFBP3

Immunogen Range: 186-292/292


Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 3486

Swiss Prot: P17936

Source: KLH conjugated synthetic peptide derived from human IGFBP3

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Background:

IGF-binding proteins prolong the half-life of the IGFs and have been shown to either inhibit or stimulate the growth promoting effects of the IGFs on cell culture. They alter the interaction of IGFs with their cell surface receptors. Also exhibits IGF-independent antiproliferative and apoptotic effects mediated by its receptor TMEM219/IGFBP-3R.

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)
ELISA(1:500-1000)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)

Predicted Molecular Weight: 30-35


Cross Reactive Species: Human
Rat

Predicted Cross Reactive Species: Mouse

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Yuan, Qing, et al. "Docetaxel-loaded solid lipid nanoparticles suppress breast cancer cells growth with reduced myelosuppression toxicity." International Journal of Nanomedicine 9 (2014): 4829.Read more>>
  • Liu et al. Effects of insulin-like growth factor binding protein 3 on apoptosis of cutaneous squamous cell carcinoma cells. (2018) Onco.Targets.Ther. 11:6569-6577Read more>>
  • Diao S et al. IGF2 enhanced the osteo‐/dentinogenic and neurogenic differentiation potentials of stem cells from apical papilla. J Oral Rehabil. 2019 Jul 10. Read more>>
  • Suwei Chen. et al. Insulin\like growth factor\binding protein 3 inhibits angiotensin II\induced aortic smooth muscle cell phenotypic switch and matrix metalloproteinase expression. Exp Physiol. 2020 Nov;105(11):1827-1839Read more>>
  • Juil Kim. et al. Mucosal ribosomal stress-induced PRDM1 promotes chemoresistance via stemness regulation. Commun Biol. 2021 May;4(1):1-14Read more>>
VALIDATION IMAGES

Formalin-fixed and paraffin embedded rat brain tissue labeled Anti-IGFBP3 Polyclonal Antibody, Unconjugated (bs-1434R) at 1:200, followed by conjugation to the secondary antibody and DAB staining


Formalin-fixed and paraffin-embedded rat brain labeled with Anti-IGFBP3 Polyclonal Antibody, Unconjugated(bs-1434R) 1:200, overnight at 4°C, The secondary antibody was Goat Anti-Rabbit IgG,Cy3 conjugated(bs-0295G-Cy3)used at 1:200 dilution for 40 minutes at 37°C.


Lane 1: HepG2 cell lysates probed with IGFBP3 Polyclonal Antibody, Unconjugated (bs-1434R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.