DATASHEET
Host:
Rabbit
Target Protein:
RKIP/PBP
Immunogen Range:
50-100/187
Clonality:
Polyclonal
Isotype:
IgG
Entrez Gene:
5037
Swiss Prot:
P30086
Source:
KLH conjugated synthetic peptide derived from human RKIP
Purification:
Purified by Protein A.
Storage Buffer:
0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage:
Store at -20°C for 12 months.
Background:
Binds ATP, opioids and phosphatidylethanolamine. Has lower affinity for phosphatidylinositol and phosphatidylcholine. Serine protease inhibitor which inhibits thrombin, neuropsin and chymotrypsin but not trypsin, tissue type plasminogen activator and elastase (By similarity). Inhibits the kinase activity of RAF1 by inhibiting its activation and by dissociating the RAF1/MEK complex and acting as a competitive inhibitor of MEK phosphorylation. HCNP may be involved in the function of the presynaptic cholinergic neurons of the central nervous system. HCNP increases the production of choline acetyltransferase but not acetylcholinesterase. Seems to be mediated by a specific receptor (By similarity).
PRODUCT SPECIFIC PUBLICATIONS
- Wei et al. Promoter methylation and expression of Raf kinase inhibitory protein in esophageal squamous cell carcinoma. (2017) Oncol.Lett. 13:1866-1872Read more>>
- Ping et al. Expression of RKIP, E-cadherin and NF-kB p65 in esophageal squamous cell carcinoma and their correlations. (2015) Int.J.Clin.Exp.Pathol. 8:10164-70Read more>>
VALIDATION IMAGES
Paraformaldehyde-fixed, paraffin embedded Rat brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with RKIP/PBP Polyclonal Antibody, Unconjugated (bs-1436R) at 1:200 overnight at 4°C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded (rat adrenal gland); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Incubation with (RKIP/PBP ) Polyclonal Antibody, Unconjugated (bs-1436R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.