bs-14399R [Primary Antibody]
phospho-Dnmt3a (Ser390 + Ser393) Polyclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: phospho-Dnmt3a (Ser390 + Ser393)

Modification Site: Ser390 + Ser393

Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 1788

Swiss Prot: Q9Y6K1

Source: KLH conjugated Synthesised phosphopeptide derived from human Dnmt3a around the phosphorylation site of Ser390 + Ser393

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.

Background:

Required for genome wide de novo methylation and is essential for the establishment of DNA methylation patterns during development. DNA methylation is coordinated with methylation of histones. It modifies DNA in a non-processive manner and also methylates non-CpG sites. May preferentially methylate DNA linker between 2 nucleosomal cores and is inhibited by histone H1. Plays a role in paternal and maternal imprinting. Required for methylation of most imprinted loci in germ cells. Acts as a transcriptional corepressor for ZNF238. Can actively repress transcription through the recruitment of HDAC activity.

Size: 100ul

Concentration: 1ug/ul

Applications: WB(WB=1:500-2000)
IHC-P(IHC-P=1:100-500)
IHC-F(IHC-F=1:100-500)
IF(IF=1:100-500)

Predicted Molecular Weight: 102


Cross Reactive Species: Human

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Paraformaldehyde-fixed, paraffin embedded Human stomach; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with phospho-Dnmt3a (Ser390 + Ser393) Polyclonal Antibody, Unconjugated (bs-14399R) at 1:200 overnight at 4°C, DAB staining.


Lane 1: 293T cell lysates; Lane 2: SH-SY5Y cell lysates; Lane 3: Molt-4 cell lysates; Lane 4: HepG2 cell lysates probed with phospho-Dnmt3a (Ser390 + Ser393) Polyclonal Antibody, Unconjugated (bs-14399R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.