bs-14399R-Cy3

DATASHEET

Host: Rabbit

Target Protein: phospho-Dnmt3a (Ser390 + Ser393)

Modification Site: Ser390 + Ser393

Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 1788

Swiss Prot: Q9Y6K1

Source: KLH conjugated Synthesised phosphopeptide derived from human Dnmt3a around the phosphorylation site of Ser390 + Ser393

Purification: Purified by Protein A.

Storage Buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.

Storage: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Background:

Required for genome wide de novo methylation and is essential for the establishment of DNA methylation patterns during development. DNA methylation is coordinated with methylation of histones. It modifies DNA in a non-processive manner and also methylates non-CpG sites. May preferentially methylate DNA linker between 2 nucleosomal cores and is inhibited by histone H1. Plays a role in paternal and maternal imprinting. Required for methylation of most imprinted loci in germ cells. Acts as a transcriptional corepressor for ZNF238. Can actively repress transcription through the recruitment of HDAC activity.

Conjugation: Cy3

Excitation/ Emission: 512,550nm/570,615nm

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)

Predicted Molecular Weight: 102

Cross Reactive Species: Human

For research use only. Not intended for diagnostic or therapeutic use.