Host: Rabbit
Target Protein: TLR3
Immunogen Range: 801-905/905
Clonality: Polyclonal
Isotype: IgG
Entrez Gene: 142980
Swiss Prot: Q99MB1
Source: KLH conjugated synthetic peptide derived from mouse TLR3
Purification: Purified by Protein A.
Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Background:
Size: 100ul
Concentration: 1ug/ul
Applications:
WB(1:300-5000)
ELISA(1:500-1000)
FCM(1:20-100)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)
Predicted Molecular Weight: 100
Cross Reactive Species:
Human
Mouse
Rat
Predicted Cross Reactive Species:
Dog
Pig
For research use only. Not intended for diagnostic or therapeutic use.
- Zhu, W., et al. "Overexpression of Toll?\Like Receptor 3 in Spleen is Associated with Experimental Arthritis in Rats."Scandinavian Journal of Immunology 76.3 (2012): 263-270..Read more>>
- Wang, Shaolan, et al. "Xenobiotic receptor PXR regulates innate immunity via activation of NLRP3 inflammasome in vascular endothelial cells." Journal of Biological Chemistry (2014): jbc-M114.Read more>>
- Zhu, Wenhua, et al. "Pristane induces autophagy in macrophages, promoting a STAT1-IRF1-TLR3 pathway and arthritis." Clinical Immunology (2016).Read more>>
- Milara et al. Roflumilast improves corticosteroid resistance COPD bronchial epithelial cells stimulated with toll like receptor 3 agonist. (2015) Respir.Res. 16:12Read more>>
- Zhao R et al. Activation of Toll-like receptor 3 promotes pathological corneal neovascularization by enhancement of SDF-1-mediated endothelial progenitor cell recruitment. (2018) Exp Eye Res. 178:177-185.Read more>>
- Zou Xiong. et al. Toll-Like Receptors Serve as Biomarkers for Early Diagnosis and Prognosis Assessment of Kidney Renal Clear Cell Carcinoma by Influencing the Immune Microenvironment: Comprehensive Bioinformatics Analysis Combined With Experimental Validation. Front Mol Biosci. 2022 Jan;0:24Read more>>
- Shasha Liu. et al. STAT3 regulates antiviral immunity by suppressing excessive interferon signaling. CELL REP. 2023 Jul;42:112806Read more>>
Paraformaldehyde-fixed, paraffin embedded rat kidney tissue; Antigen retrieval by boiling in sodium citrate buffer(pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37°C for 20min; Antibody incubation with Rabbit Anti-TLR3 Polyclonal Antibody, Unconjugated (bs-1444R) at 1:400 overnight at 4°C, followed by a conjugated secondary and DAB staining
Paraformaldehyde-fixed, paraffin embedded rat brain tissue; Antigen retrieval by boiling in sodium citrate buffer(pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37°C for 20min; Antibody incubation with Rabbit Anti-TLR3 Polyclonal Antibody, Unconjugated (bs-1444R) at 1:400 overnight at 4°C, followed by a conjugated secondary and DAB staining
Lane 1: Mouse Placenta lysates; Lane 2: Human Jurkat cell lysates; Lane 3: Human Hela cell lysates; Lane 4: Human MOLT-4 cell lysates probed with TLR3 Polyclonal Antibody, Unconjugated (bs-1444R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.