bs-14459R [Primary Antibody]
DUSP26 Polyclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: DUSP26

Immunogen Range: 111-211/211


Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 78986

Swiss Prot: Q9BV47

Source: KLH conjugated synthetic peptide derived from human DUSP26

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Background:

Mitogen-activated protein (MAP) kinases are a large class of proteins involved in signal transduction pathways, which are activated by a range of stimuli and mediate a number of physiological and pathological changes in the cell. Dual specificity phosphatases (DUSPs) are a subclass of the protein tyrosine phosphatase (PTP) gene superfamily, which are selective for dephosphorylating critical phosphothreonine and phosphotyrosine residues within MAP kinases. DUSP gene expression is induced by a host of growth factors and/or cellular stresses, thereby negatively regulating MAP kinase superfamily members including MAPK/ERK, SAPK/JNK and p38. DUSP26, also designated LDP4, MKP8, NATA1 and SKRP3, is ubiquitously expressed in brain except in the hippocampus. DUSP26 dephosphorylates p38 thereby inhibiting p38-mediated apoptosis in anaplastic thyroid cancer cells. Downregulation of DUSP26 may also contribute to malignant phenotypes of glioma.

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)
ELISA(1:500-1000)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)
ICC(1:100-500)

Predicted Molecular Weight: 24


Cross Reactive Species: Rat

Predicted Cross Reactive Species: Human
Mouse
Cow
Pig
Horse
Rabbit

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Paraformaldehyde-fixed, paraffin embedded Rat brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with DUSP26 Polyclonal Antibody, Unconjugated (bs-14459R) at 1:400 overnight at 4°C, DAB staining.