bs-1448R
[Primary Antibody]
Jagged1 Polyclonal Antibody
DATASHEET
Host: Rabbit
Target Protein: Jagged1
Clonality: Polyclonal
Isotype: IgG
Entrez Gene: 182
Source: KLH conjugated synthetic peptide derived from human Jagged1
Purification: Purified by Protein A.
Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage: Store at -20°C for 12 months.
Background:
The jagged 1 protein encoded by JAG1 is the human homolog of the Drosophilia jagged protein. Human jagged 1 is the ligand for the receptor notch 1, the latter a human homolog of the Drosophilia jagged receptor notch. Mutations that alter the jagged 1 protein cause Alagille syndrome. Jagged 1 signalling through notch 1 has also been shown to play a role in hematopoiesis. [provided by RefSeq].
Size: 100ul
Concentration: 1ug/ul
Applications:
WB(1:300-5000)
IHC-P(1:200-400)
IF(IHC-P)(1:50-200)
IF(ICC)(1:50-200)
Cross Reactive Species:
Human
Mouse
Rat
For research use only. Not intended for diagnostic or therapeutic use.
PRODUCT SPECIFIC PUBLICATIONS
- Wang et al. Lycium barbarum Polysaccharide Promotes Maturation of Dendritic Cell via Notch Signaling and Strengthens Dendritic Cell Mediated T Lymphocyte Cytotoxicity on Colon Cancer Cell CT26-WT. (2018) Evid.Based.Complement.Alternat.Med. 2018:2305683Read more>>
- Deng S et al. The regulatory roles of VEGF-Notch signaling pathway on aplastic anemia with kidney deficiency and blood stasis. (2018) Journal of Cellular Biochemistry. Read more>>
- Zhang Y et al. The Notch signaling pathway inhibitor Dapt alleviates autism-like behavior, autophagy and dendritic spine density abnormalities in a valproic acid-induced animal model of autism. Prog Neuropsychopharmacol Biol Psychiatry. 2019 May 7;94:109644. Read more>>
- Xiangxun Chen. et al. Combination of Sodium Cantharidinate with Cisplatin Synergistically Hampers Growth of Cervical Cancer. Drug Des Dev Ther. 2021; 15: 171C183Read more>>
- Jianfei Shi. et al. Matrine promotes hepatic oval cells differentiation into hepatocytes and alleviates liver injury by suppression of Notch signalling pathway. Life Sci. 2020 Nov;261:118354Read more>>
- Fang-Fang CHEN. et al. Tao-Hong-Si-Wu Decoction promotes angiogenesis after cerebral ischaemia in rats via platelet microparticles. Chin J Nat Medicines. 2020 Aug;18:62Read more>>
- Yating Li. et al. Effects of Notch2 on proliferation, apoptosis and steroidogenesis in bovine luteinized granulosa cells. Theriogenology. 2021 Sep;171:55Read more>>
- Xuerui Hu. et al. The Effect of Poria cocos Polysaccharide PCP-1C on M1 Macrophage Polarization via the Notch Signaling Pathway. MOLECULES. 2023 Jan;28(5):214Read more>>
- Ying-Hua Zhang. et al. N-acetylcysteine improves autism-like behavior by recovering autophagic deficiency and decreasing Notch-1/Hes-1 pathway activity. EXP BIOL MED. ;()Read more>>
- Qing Zhang. et al. Cancer-associated fibroblasts-induced remodeling of tumor immune microenvironment via Jagged1 in glioma. CELL SIGNAL. 2024 Mar;115:111016Read more>>
- Wang Kun. et al. Modulating Th1/Th2 drift in asthma-related immune inflammation by enhancing bone mesenchymal stem cell homing through targeted inhibition of the Notch1/Jagged1 signaling pathway. INT IMMUNOPHARMACOL. 2024 Mar;130:111713Read more>>
VALIDATION IMAGES
Formalin-fixed and paraffin embedded mouse colon labeled with Anti-Jagged1/CD339 Polyclonal Antibody, Unconjugated (bs-1448R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.
HepG2 cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37°C for 20 min; Antibody incubation with (Jagged1) polyclonal Antibody, Unconjugated (bs-1448R) 1:100, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.
Lane 1: Recombinant human CD339 protein, N-His(bs-42225P) probed with Jagged1 Polyclonal Antibody, Unconjugated (bs-1448R) at 1:500 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.