bs-1453R [Primary Antibody]
Caveolin-1 Polyclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: Caveolin-1

Immunogen Range: 2-120/178


Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 857

Swiss Prot: Q03135

Source: KLH conjugated synthetic peptide derived from human Caveolin-1

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Background:

May act as a scaffolding protein within caveolar membranes. Interacts directly with G-protein alpha subunits and can functionally regulate their activity (By similarity). Involved in the costimulatory signal essential for T-cell receptor (TCR)-mediated T-cell activation. Its binding to DPP4 induces T-cell proliferation and NF-kappa-B activation in a T-cell receptor/CD3-dependent manner. Recruits CTNNB1 to caveolar membranes and may regulate CTNNB1-mediated signaling through the Wnt pathway.

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)
ELISA(1:500-1000)
FCM(1:20-100)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)

Predicted Molecular Weight: 20


Cross Reactive Species: Human
Mouse
Rat

Predicted Cross Reactive Species: Dog
Cow
Sheep
Pig
Horse
Rabbit

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Cheng, Mao-Feng, et al. "The role of rosiglitazone in the proliferation of vascular smooth muscle cells after experimental subarachnoid hemorrhage." Acta Neurochirurgica (2014): 1-7.Read more>>
  • Dixit, Saurabh, et al. "Caveolin-mediated endocytosis of the Chlamydia M278 outer membrane peptide encapsulated in poly (lactic acid)-Poly (ethylene glycol) nanoparticles by mouse primary dendritic cells enhances specific immune effectors mediated by MHC class II and CD4+ T cells." Biomaterials (2017).Read more>>
  • Zhang et al. P62 regulates resveratrol-mediated Fas/Cav-1 complex formation and transition from autophagy to apoptosis. (2015) Oncotarge. 6:789-801Read more>>
  • Cheng S et al. Comparative analysis of Longissimus dorsi tissue from two sheep groups identifies differentially expressed genes related to growth, development and meat quality. Genomics. 2020 Sep;112(5):3322-3330.Read more>>
  • Zhang Q et al. CTRP13 attenuates the expression of LN and CAV-1 Induced by high glucose via CaMKK/AMPK pathway in rLSECs. Aging (Albany NY). 2020 Jun 17;12(12):11485-11499.Read more>>
  • Xian J et al. Progress in the research of p53 tumour suppressor activity controlled by Numb in triple\negative breast cancer. J Cell Mol Med. 2020 Jul;24(13):7451-7459.Read more>>
  • Hao Chen. et al. MicroRNA-124-3p affects myogenic differentiation of adipose-derived stem cells by targeting Caveolin-1 during pelvic floor dysfunction in Sprague Dawley rats. Ann Transl Med. 2021 Jan; 9(2): 161Read more>>
  • Quanwei Zhang. et al. DIA proteomics identified the potential targets associated with angiogenesis in the mammary glands of dairy cows with hemorrhagic mastitis. FRONT VET SCI. 2022; 9: 980963Read more>>
  • Yingna Huang. et al. Nisoldipine Inhibits Influenza A Virus Infection by Interfering with Virus Internalization Process. VIRUSES-BASEL. 2022 Dec;14(12):2738Read more>>
  • Hai-Xia Li. et al. The purified extract of steamed Panax ginseng protects cardiomyocyte from ischemic injury via caveolin-1 phosphorylation-mediating calcium influx. J GINSENG RES. 2023 AugRead more>>
  • in Ma. et al. Down-regulation of COL1A1 inhibits tumor-associated fibroblast activation and mediates matrix remodeling in the tumor microenvironment of breast cancer. OPEN LIFE SCI. 2023 Jan;18(1)Read more>>
VALIDATION IMAGES

Formalin-fixed and paraffin embedded human lung carcinoma labeled with Anti-Caveolin-1 Polyclonal Antibody, Unconjugated (bs-1453R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.


Formalin-fixed and paraffin embedded mouse eye labeled with Anti Caveolin-1 Polyclonal Antibody, Unconjugated (bs-1453R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.


RSC96 cells probed with Caveolin-1 Polyclonal Antibody, Unconjugated (bs-1453R) at 1:100 for 30 minutes followed by incubation with a conjugated secondary (PE Conjugated) (green) for 30 minutes compared to control cells (blue), secondary only (light blue) and isotype control (orange).


Lane 1: mouse lung lysates Lane 2: mouse muscle lysates probed with Anti Caveolin-1 Polyclonal Antibody, Unconjugated (bs-1453R) at 1:200 in 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 90min in 37˚C. Predicted band 20kD. Observed band size: 20kD.


Lane 1: Lung lysates probed with Caveolin-1 Polyclonal Antibody, Unconjugated (bs-1453R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.