bs-14574R [Primary Antibody]
ELP4 Polyclonal Antibody
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Host: Rabbit

Target Protein: ELP4

Immunogen Range: 321-424/424


Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 26610

Swiss Prot: Q96EB1

Source: KLH conjugated synthetic peptide derived from human ELP4

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.

Background:

Acts as subunit of the RNA polymerase II elongator complex, which is a histone acetyltransferase component of the RNA polymerase II (Pol II) holoenzyme and is involved in transcriptional elongation. Elongator may play a role in chromatin remodeling and is involved in acetylation of histones H3 and probably H4.

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)
ELISA(1:500-1000)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)

Predicted Molecular Weight: 47


Cross Reactive Species: Human
Mouse
Rat

Predicted Cross Reactive Species: Rabbit

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

HeLa cell lysates probed with Rabbit Anti-ELP4 Polyclonal Antibody, Unconjugated (bs-14574R) at 1:300 in 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:5000 for 90min at RT.


Paraformaldehyde-fixed, paraffin embedded Rat brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with ELP4 Polyclonal Antibody, Unconjugated (bs-14574R) at 1:200 overnight at 4°C, DAB staining.


Lane 1: Mouse Cerebrum tissue lysates; Lane 2: Mouse Prostate tissue lysates; Lane 3: Rat Stomach tissue lysates; Lane 4: Rat Cerebrum tissue lysates probed with ELP4 Polyclonal Antibody, Unconjugated (bs-14574R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at room temperature for 60 min.