Host: Rabbit
Target Protein: LIFR/CD118
Immunogen Range: 451-580/1093
Clonality: Polyclonal
Isotype: IgG
Entrez Gene: 3977
Swiss Prot: O70535
Source: KLH conjugated synthetic peptide derived from rat LIFR
Purification: Purified by Protein A.
Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Background:
Size: 100ul
Concentration: 1ug/ul
Applications:
WB(1:300-5000)
ELISA(1:500-1000)
FCM(1:20-100)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)
Predicted Molecular Weight: 117
Cross Reactive Species:
Human
Others
Predicted Cross Reactive Species:
Mouse
Rat
Dog
Cow
For research use only. Not intended for diagnostic or therapeutic use.
- Murphy, Melinda J., et al. "Leukemia Inhibitory Factor is Necessary for Ovulation in Female Rhesus Macaques." Endocrinology (2016): en-2016.Read more>>
- Lakshana Sreenivasan. et al. Targeting the gp130/STAT3 Axis Attenuates Tumor Microenvironment Mediated Chemoresistance in Group 3 Medulloblastoma Cells. Cells-Basel. 2022 Jan;11(3):381Read more>>
- in Ma. et al. IFNT-induced IRF1 enhances bovine endometrial receptivity by transactivating LIFR. J REPROD IMMUNOL. 2024 Feb;:104212Read more>>
Formalin-fixed and paraffin-embedded human colon carcinoma labeled with Rabbit Anti-CD118 Polyclonal Antibody (bs-1458R), Unconjugated 1:200 followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin embedded human breast carcinoma labeled with Anti LIFR/CD118 Polyclonal Antibody, Unconjugated (bs-1458R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Human U937 cells probed with LIFR/CD118 Polyclonal Antibody, Unconjugated (bs-1458R) (green) at 1:100 for 30 minutes followed by a PE conjugated secondary antibody compared to unstained cells (blue), secondary only (light blue), and isotype control (orange).
MCF-7 lysates probed with LIFR/CD118 Polyclonal Antibody, Unconjugated (bs-1458R) at 1:300 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C
Paraformaldehyde-fixed, paraffin embedded Rat muscle; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with LIFR/CD118 Polyclonal Antibody, Unconjugated (bs-1458R) at 1:200 overnight at 4°C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Rat brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with LIFR/CD118 Polyclonal Antibody, Unconjugated (bs-1458R) at 1:200 overnight at 4°C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Rat placenta; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with LIFR/CD118 Polyclonal Antibody, Unconjugated (bs-1458R) at 1:200 overnight at 4°C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Mouse brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with LIFR/CD118 Polyclonal Antibody, Unconjugated (bs-1458R) at 1:200 overnight at 4°C, DAB staining.
Lane 1: Human U-87 MG cell Lysates; Lane 2: Human U2OS cell Lysates; Lane 3: Human HUVEC cell Lysates; Lane 4: Human SH-SY5Y cell Lysates. Probed with LIFR polyclonal Antibody, unconjugated (bs-1458R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.