bs-1461R [Primary Antibody]
VDAC1 Polyclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: VDAC

Specificity: May detect VDAC2 due to sequence similarity

Immunogen Range: 85-190/283


Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 7416

Swiss Prot: P21796

Source: KLH conjugated synthetic peptide derived from human VDAC1

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.

Storage: Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.

Background:

Forms a channel through the mitochondrial outer membrane and also the plasma membrane. The channel at the outer mitochondrial membrane allows diffusion of small hydrophilic molecules; in the plasma membrane it is involved in cell volume regulation and apoptosis. It adopts an open conformation at low or zero membrane potential and a closed conformation at potentials above 30-40 mV. The open state has a weak anion selectivity whereas the closed state is cation-selective. May participate in the formation of the permeability transition pore complex (PTPC) responsible for the release of mitochondrial products that triggers apoptosis.

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)
ELISA(1:500-1000)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)

Predicted Molecular Weight: 32


Cross Reactive Species: Human
Rat

Predicted Cross Reactive Species: Mouse
Dog
Cow
Sheep
Pig
Horse
Rabbit

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Manczak, Maria, and P. Hemachandra Reddy. "Abnormal interaction of VDAC1 with amyloid beta and phosphorylated tau causes mitochondrial dysfunction in Alzheimer's disease." Human molecular genetics 21.23 (2012): 5131-5146.Read more>>
  • Xing, Wen Min, et al. "Proteomic identification of mitochondrial targets involved in andrographolide sodium bisulfite-induced nephrotoxicity in a rat model."Environmental Toxicology and Pharmacology (2015).Read more>>
  • Wang, Zhaoqi, et al. "Penehyclidine hydrochloride prevents anoxia/reoxygenation injury and induces H9c2 cardiomyocyte apoptosis via a mitochondrial pathway." European Journal of Pharmacology (2017).Read more>>
  • Hui, Yan, et al. "Resveratrol improves mitochondrial function in the remnant kidney from 5/6 nephrectomized rats." Acta Histochemica (2017).Read more>>
  • Tan, Hongbao, Li Chen, and Jun Ma. "Penehyclidine hydrochloride post‑conditioning reduces ischemia/reperfusion‑induced cardiomyocyte apoptosis in rats." Experimental and Therapeutic Medicine.Read more>>
  • Tan et al. Penehyclidine hydrochloride post-conditioning reduces ischemia/reperfusion-induced cardiomyocyte apoptosis in rats. (2017) Exp.Ther.Me. 14:4272-4278Read more>>
VALIDATION IMAGES

Formalin-fixed and paraffin embedded rat brain labeled with Rabbit Anti VDAC Polyclonal Antibody, Unconjugated (bs-1461R) at 1:200 followed by conjugation to the secondary antibody and DAB staining