Host: Rabbit
Target Protein: ERp57
Specificity: This antibody may have slight cross reactivity with ZFYVE26 and DCLRE1C due to partial sequence similarity
Immunogen Range: 351-450/505
Clonality: Polyclonal
Isotype: IgG
Entrez Gene: 2923
Swiss Prot: P30101
Source: KLH conjugated synthetic peptide derived from human ERp57
Purification: Purified by Protein A.
Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Background:
Size: 100ul
Concentration: 1ug/ul
Applications:
WB(1:300-5000)
ELISA(1:500-1000)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)
ICC(1:100-500)
Predicted Molecular Weight: 54
Cross Reactive Species:
Mouse
Rat
Predicted Cross Reactive Species:
Human
Chicken
Rabbit
For research use only. Not intended for diagnostic or therapeutic use.
- Yang W et al. The role of protein disulphide-isomerase A3 as autoantigen in the pathogenesis of autoimmune thyroiditis and related brain damage in adult mice. Clin Immunol. 2020 Jan 23;212:108350. Read more>>
Mouse embryo lysates probed with ERp57 Polyclonal Antibody, Unconjugated (bs-14629R) at 1:300 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:10000 for 60 min at 37˚C.
Lane 1: Mouse Skin tissue lysates; Lane 2: Mouse Testis tissue lysates; Lane 3: Rat Placenta tissue lysates; Lane 4: Rat Testis tissue lysates; Lane 5: Human A375 cell lysates; Lane 6: Human HepG2 cell lysates; Lane 7: Human HeLa cell lysates probed with ERp57 Polyclonal Antibody, Unconjugated (bs-14629R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at room temperature for 60 min.