bs-1464R [Primary Antibody]
GnRHR Polyclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: GnRHR

Immunogen Range: 201-328/328


Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 2798

Swiss Prot: P30968

Source: KLH conjugated synthetic peptide derived from human GnRHR

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Background:

Receptor for gonadotropin releasing hormone (GnRH) that mediates the action of GnRH to stimulate the secretion of the gonadotropic hormones luteinizing hormone (LH) and follicle-stimulating hormone (FSH). This receptor mediates its action by association with G-proteins that activate a phosphatidylinositol-calcium second messenger system. Isoform 2 may act as an inhibitor of GnRH-R signaling.

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)
ELISA(1:500-1000)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)
ICC(1:100-500)

Predicted Molecular Weight: 36


Cross Reactive Species: Human
Mouse
Rat

Predicted Cross Reactive Species: Dog
Cow
Sheep
Pig
Horse
Rabbit

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Xiao, Liu, et al. "Distribution patterns and developmental changes of GnRH and GnRHR-immunopositive cells in the pituitary of Ji Ning gray goats." Pakistan Veterinary Journal 34.1 (2014): 116-119.Read more>>
  • Liu, Te, et al. "Effects of di-(2-ethylhexyl) phthalate on the hypothalamus-pituitary-ovarian axis in adult female rats." Reproductive Toxicology (2014).Read more>>
  • López-Doval, S., R. Salgado, and A. Lafuente. "The expression of several reproductive hormone receptors can be modified by perfluorooctane sulfonate (PFOS) in adult male rats." Chemosphere 155 (2016): 488-497.Read more>>
  • Lu Xiaosheng. et al. Deficiency of C1QL1 reduced murine ovarian follicle reserve through intraovarian and endocrine control. ENDOCRINOLOGY. 2022 ApRead more>>
  • Zixuan Chen. et al. Therapeutic Effects of Melatonin in Female Mice with Central Precocious Puberty by Regulating the Hypothalamic Kiss-1/Kiss1R System. BEHAV BRAIN RES. 2023 Nov;:114783Read more>>
VALIDATION IMAGES

U251 lysates probed with GnRHR Polyclonal Antibody, unconjugated (bs-1464R) at 1:300 overnight at 4°C followed by a conjugated secondary antibody at 1:10000 for 60 minutes at 37°C.


Formalin-fixed and paraffin embedded rat brain labeled with Anti-GnRHR Polyclonal Antibody, Unconjugated (bs-1464R) at 1:200 followed by conjugation to the secondary antibody and DAB staining


Formalin-fixed and paraffin-embedded rat brain labeled with Rabbit Anti-GnRHR Polyclonal Antibody, Unconjugated(bs-1464R) 1:200, overnight at 4°C, The secondary antibody was Goat Anti-Rabbit IgG, Cy3 conjugated(bs-0295G-Cy3)used at 1:200 dilution for 40 minutes at 37°C.


Lane 1: Mouse Cerebrum lysates; Lane 2: Jurkat cell lysates probed with GnRHR Polyclonal Antibody, Unconjugated (bs-1464R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.