bs-1468R [Primary Antibody]
MDR1 Polyclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: MDR1

Immunogen Range: 1051-1280/1280


Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 5243

Source: KLH conjugated synthetic peptide derived from human MDR1

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Background:

P Glycoprotein, the product of the MDR1 gene, is expressed in distinct non-malignant cells, typically cells with secretory and excretory functions. It is assumed to function as an ATP-dependent drug efflux pump with broad substrate specificity. The highest expression of P Glycoprotein has been observed in kidney (proximal tubules), liver (bile canaliculi), adrenal gland and intestine, suggesting that the primary role of P Glycoprotein is in the normal secretion of physiological metabolites and ingested chemicals into bile, urine and the lumen of the intestinal tract. Elevated levels of P Glycoprotein have also been reported in multidrug-resistant cell lines and in colon, endometrial, ovarian, and breast tumors, as well as in sarcomas and leukemias / lymphomas.

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)
ELISA(1:500-1000)
FCM(1:20-100)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)

Predicted Molecular Weight: 141


Cross Reactive Species: Human
Mouse
Rat

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Zhang E et al. 7-O-geranylquercetin contributes to reverse P-gp-mediated adriamycin resistance in breast cancer. Life Sci. 2019 Oct 5:116938. Read more>>
  • Du J et al. MAY, a novel tubulin inhibitor, induces cell apoptosis in A549 and A549/Taxol cells and inhibits epithelial-mesenchymal transition in A549/Taxol cells. Chem Biol Interact. 2020 May 25;323:109074.Read more>>
  • Zhao H et al. Gli affects the stemness and prognosis of epithelial ovarian cancer via homeobox protein NANOGMol Med Rep.2021 Feb;23(2):128.Read more>>
  • Jiameng Qu. et al. Induction of P-glycoprotein expression by dandelion in tumor and heart tissues: Impact on the anti-tumor activity and cardiotoxicity of doxorubicin. PHYTOMEDICINE. 2022 Jun;:154275Read more>>
  • Zipeng Gong. et al. Pharmacokinetic Differences of Wuji Pill Components in Normal and Chronic Visceral Hypersensitivity Irritable Bowel Syndrome Rats Attributable to Changes in Tight Junction and Transporters. FRONT PHARMACOL. 2022; 13: 948678Read more>>
  • Huang, Wenjie. et al. The inhibitory effect and mechanism of Yi-qi-hua-yu-jie-du decoction on the drug resistance of gastric cancer stem cells based on ABC transporters. CHIN MED-UK. 2022 Dec;17(1):1-18Read more>>
  • Danyu Wang. et al. Photoactivated DNA Nanodrugs Damage Mitochondria to Improve Gene Therapy for Reversing Chemoresistance. ACS NANO. 2023;XXXX(XXX):XXX-XXXRead more>>
VALIDATION IMAGES

Tissue/cell:SH-SY5Y cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum,C-0005) at 37°C for 20 min; Antibody incubation with (MDR1/P Glycoprotein) polyclonal Antibody, Unconjugated (bs-1468R) 1:100, 90 minutes at 37°C; followed by a FITC conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei