bs-1502R [Primary Antibody]
CXCL10 Polyclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: CXCL10

Immunogen Range: 35-98/98

Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 15945

Swiss Prot: P17515

Source: KLH conjugated synthetic peptide derived from mouse CXCL10

Purification: Purified by Protein A.

Storage Buffer: Aqueous buffered solution containing 1% BSA, 50% glycerol and 0.09% sodium azide.

Storage Condition: Store at -20°C for 12 months..

Background: In addition to its role as a proinflammatory cytokine, may participate in T-cell effector function and perhaps T-cell development.

Size: 100ul

Concentration: 1ug/ul

Applications: ELISA(1:500-1000)
FCM(1:20-100)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)

Cross Reactive Species: Human
Mouse
Rat

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Li, Yu-Tung, Tomoyuki Nishikawa, and Yasufumi Kaneda. "Platelet-cytokine Complex Suppresses Tumour Growth by Exploiting Intratumoural Thrombin-dependent Platelet Aggregation." Scientific Reports 6 (2016): 25077.Read more>>
  • Djamiatun, Kis, et al. "Annona muricata modulate brain-CXCL10 expression during cerebral malaria phase." IOP Conference Series: Earth and Environmental Science. Vol. 55. No. 1. IOP Publishing, 2017.Read more>>
  • Iversen, Marie B., et al. "An innate antiviral pathway acting before interferons at epithelial surfaces." Nature immunology (2015).Read more>>
  • Clancy-Thompson, Eleanor, et al. "Melanoma induces, and adenosine suppresses, CXCR3-cognate chemokine production and T-cell infiltration of lungs bearing metastatic-like disease." Cancer immunology research 3.8 (2015): 956-967.Read more>>
  • Henschel et al. Modulation of the diet and gastrointestinal microbiota normalizes systemic inflammation and β-cell chemokine expression associated with autoimmune diabetes susceptibility. (2018) PLoS.One. 13:e0190351Read more>>
  • Lee et al. Establishment of a chronic obstructive pulmonary disease mouse model based on the elapsed time after LPS intranasal instillation. (2018) Lab.Anim.Res. 34:01-OctRead more>>
  • Günther et al. Defective removal of ribonucleotides from DNA promotes systemic autoimmunity. (2015) J.Clin.Invest. 125:413-24Read more>>
  • Kang et al. Tissue-Specific Regulation of p38α-Mediated Inflammation in Con A-Induced Acute Liver Damage. (2015) J.Immunol. 194:4759-66Read more>>
  • Yang R et al. Cordycepin Attenuates IFN-γ-Induced Macrophage IP-10 and Mig Expressions by Inhibiting STAT1 Activity in CFA-Induced Inflammation Mice Model. Inflammation. 2019 Dec 23.Read more>>
  • Manuel Z et al. Blocking PDGF-CC signaling ameliorates multiple sclerosis-like neuroinflammation by inhibiting disruption of the bloodCbrain barrierSci Rep.2020 Dec 24;10(1):22383.Read more>>
  • Hu Sun. et al. Study on the relationship between CXCR3 and its ligands and tubal tuberculosis. Life Sci. 2021 May;272:119047Read more>>
VALIDATION IMAGES

Formalin-fixed and paraffin embedded rat lung labeled with Anti-CXCL10 Polyclonal Antibody, Unconjugated (bs-1502R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.


Raw264.7 cells were fixed with 4% PFA for 10min at room temperature,permeabilized with 0.1% PBST for 20 min at room temperature, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with CXCL10 Polyclonal Antibody(bs-1502R)at 1:100 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).