bs-1506R
[Primary Antibody]
AQP1 Polyclonal Antibody
DATASHEET
Host: Rabbit
Target Protein: AQP1
Immunogen Range: 181-269/269
Clonality: Polyclonal
Isotype: IgG
Entrez Gene: 358
Swiss Prot: P29972
Source: KLH conjugated synthetic peptide derived from human AQP1
Purification: Purified by Protein A.
Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Background:
Forms a water-specific channel that provides the plasma membranes of red cells and kidney proximal tubules with high permeability to water, thereby permitting water to move in the direction of an osmotic gradient.
Size: 100ul
Concentration: 1ug/ul
Applications:
WB(1:300-5000)
ELISA(1:500-1000)
FCM(1:20-100)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)
Predicted Molecular Weight: 28
Cross Reactive Species:
Human
Mouse
Rat
Predicted Cross Reactive Species:
Dog
Cow
Sheep
Pig
Horse
For research use only. Not intended for diagnostic or therapeutic use.
PRODUCT SPECIFIC PUBLICATIONS
- Wang EX et al. Stem cells from trabecular meshwork cells can secrete extracellular matrix. Biochem Biophys Res Commun. 2020 Mar 5;523(2):522-526.Read more>>
- Sato K et al. Different Prognostic Implications of Aquaporin-1 and Aquaporin-5 Expression among Different Histological Types of Ovarian Carcinoma.Pathol Oncol Res. 2018 Jul 19. Read more>>
- Wang EX et al. Stem cells from trabecular meshwork cells can secrete extracellular matrix. Biochem Biophys Res Commun. 2020 Mar 5;523(2):522-526.Read more>>
- Takanori Watanabe et al. Aquaporin 3 Expression in Endometrioid Carcinoma of the Uterine Body Correlated With Early Stage and Lower Grade. Pathol Oncol Res. 2020 Oct;26(4):2247-2253.Read more>>
- Chao Lu. et al. Inhibition of Pre-B Cell Colony Enhancing Factor Reduces Lung Injury in Rats Receiving Cardiopulmonary Bypass. Drug Des Dev Ther. 2021; 15: 51C6Read more>>
- Fang-Hao Cai. et al. Diagnostic roles of urinary kidney microvesicles in diabetic nephropathy. Ann Transl Med. 2020 Nov; 8(21): 1431Read more>>
- Haifa Alkhalifa. et al. Inhibition of aquaporins as a potential adjunct to breast cancer cryotherapy. Oncol Lett. 2021 Jun;21(6):1-11Read more>>
- Mr. Aleksandr Vendrov. et al. Renal NOXA1/NOX1 Signaling Regulates Epithelial Sodium Channel and Sodium Retention in Angiotensin II-induced Hypertension. 2021 Oct 29Read more>>
- Yang, Jiashu. et al. m6A-mediated upregulation of AC008 promotes osteoarthritis progression through the miR-328-3p?AQP1/ANKH axis. Exp Mol Med. 2021 Nov;:1-12Read more>>
- Zhaoqi Zhang. et al. Central gene transcriptional regulatory networks shaping monocyte development in bone marrow.. FRONT IMMUNOL. 2022 Oct;13:1011279-1011279Read more>>
- Candan B.. et al. Beneficial Effects of Selenium on Kidney Injury via Nf-Kb and Aquaporin-1 Levels. BIOL TRACE ELEM RES. 2023 Nov;:1-9Read more>>
VALIDATION IMAGES
Paraformaldehyde-fixed, paraffin embedded rat kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with AQP1 Polyclonal Antibody, Unconjugated (bs-1506R) at 1:200 overnight at 4°C, followed by a conjugated secondary (Goat Anti-rabbit IgG/Bio) for 20minutes at 37°C, followed by a conjugated streptavidin (bs-0437P-Cy3) at 1:500 for 40 minutes and DAPI staining of the nuclei.
Paraformaldehyde-fixed, paraffin embedded human kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with AQP1 Polyclonal Antibody, Unconjugated (bs-1506R) at 1:200 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.
HeLa cells were stained with AQP1 Polyclonal Antibody, Unconjugated(bs-1506R) at 1:500 in PBS and incubated for two hours at 37°C followed by Goat Anti-Rabbit IgG (H+L) Cy3 conjugated secondary antibody. DAPI staining of the nucleus was done and then detected.
Lane 1: mouse heart lysates Lane 2: mouse lung lysates probed with Anti AQP1/CHIP Polyclonal Antibody, Unconjugated (bs-1506R) at 1:200 in 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 90min in RT. Predicted band 28kD. Observed band size: 28kD.
Human 293T cells probed with AQP1 Polyclonal Antibody, Unconjugated (bs-1506R) (green) at 1:100 for 30 minutes followed by a PE conjugated secondary antibody compared to unstained cells (blue), secondary only (light blue), and isotype control (orange).\n
Lane 1: Mouse Lung lysates probed with AQP1 Polyclonal Antibody, Unconjugated (bs-1506R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Paraformaldehyde-fixed, paraffin embedded (rat kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (AQP1) Polyclonal Antibody, Unconjugated (bs-1506R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (mouse kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (AQP1) Polyclonal Antibody, Unconjugated (bs-1506R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Lane 1: Mouse Kidney tissue lysates; Lane 2: Mouse Heart tissue lysates; Lane 3: Mouse Lung tissue lysates; Lane 4: Rat Kidney tissue lysates probed with AQP1 Polyclonal Antibody, Unconjugated (bs-1506R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.