bs-15075R [Primary Antibody]
C1orf69 Polyclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: C1orf69

Immunogen Range: 131-230/256


Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 200205

Source: KLH conjugated synthetic peptide derived from human C1orf69

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Background:

Belonging to the CAF17 subfamily of the gcvT family, C1orf69 is a 356 amino acid mitochondrial protein that is required for normal heme synthesis. Heme synthesis is dependent upon receiving iron through iron sulfur (Fe-S) cluster biogenesis proteins such as C1orf69, which is specifically thought to assemble Fe-S clusters for mitochondrial aconitase and lipoate synthase. In zebrafish, deficiency of C1orf69 causes anemia. The gene encoding C1orf69 maps to human chromosome 1, the largest human chromosome spanning about 260 million base pairs and making up 8% of the human genome. There are about 3,000 genes on chromosome 1, and considering the great number of genes there are also a large number of diseases associated with chromosome 1.

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)
ELISA(1:500-1000)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)
ICC(1:100-500)

Predicted Molecular Weight: 34


Cross Reactive Species: Rat

Predicted Cross Reactive Species: Human
Mouse
Pig

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Yang, Ling. et al. Systematic analysis of expression profiles and prognostic significance for MMDS-related ironCsulfur proteins in renal clear cell carcinoma. SCI REP-UK. 2022 Nov;12(1):1-12Read more>>
VALIDATION IMAGES

Paraformaldehyde-fixed, paraffin embedded rat kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with C1orf69 Polyclonal Antibody, Unconjugated (bs-15075R) at 1:500 overnight at 4°C, followed by a conjugated secondary for 20 minutes and DAB staining