bs-1514R [Primary Antibody]
CD33 Polyclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: CD33

Immunogen Range: 101-200/334


Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 12489

Swiss Prot: Q63994

Source: KLH conjugated synthetic peptide derived from mouse CD33

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Background:

Putative adhesion molecule of myelomonocytic-derived cells that mediates sialic-acid dependent binding to cells. Preferentially binds to alpha-2,6-linked sialic acid (By similarity). The sialic acid recognition site may be masked by cis interactions with sialic acids on the same cell surface.

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)
ELISA(1:500-1000)
FCM(1:20-100)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)
ICC(1:100-500)

Predicted Molecular Weight: 40/37


Cross Reactive Species: Human
Mouse

Predicted Cross Reactive Species: Rat

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Shi, Kejian, et al. "Sodium selenite alters microtubule assembly and induces apoptosis in vitro and in vivo." Journal of hematology & oncology 6.1 (2013): 1-9.Read more>>
  • Shan, Wulin, et al. "LukS-PV, a component of Panton-Valentine leukocidin, exerts potent activity against acute myeloid leukemia in vitro and in vivo." The International Journal of Biochemistry & Cell Biology (2015).Read more>>
  • Iwami et al. Vascular endothelial growth factor c/vascular endothelial growth factor receptor 3 signaling regulates chemokine gradients and lymphocyte migration from tissues to lymphatics. (2015) Transplantatio. 99:668-77Read more>>
  • Jiang et al. Involvement of p38 in signal switching from autophagy to apoptosis via the PERK/eIF2α/ATF4 axis in selenite-treated NB4 cells. (2014) Cell.Death.Dis. 5:e1270Read more>>
  • Katarzyna Sikorskaet al. The Impact of Ag Nanoparticles and CdTe Quantum Dots on Expression and Function of Receptors Involved in Amyloid- Uptake by BV-2 Microglial Cells. Materials (Basel). 2020 Jul 20;13(14):3227.Read more>>
VALIDATION IMAGES

Mouse splenocytes probed with CD33 Polyclonal Antibody, Unconjugated (bs-1514R) at 1:100 for 30 minutes followed by incubation with a PE Conjugated secondary (green) for 30 minutes compared to control cells (blue), secondary only (light blue) and isotype control (orange).


MCK-7 lysates probed with CD33 Polyclonal Antibody, Unconjugated (bs-1514R) at 1:300 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C