VALIDATION IMAGES
Formalin-fixed and paraffin embedded mouse kidney labeled with Rabbit Anti E cadherin/CD324 Polyclonal Antibody, Unconjugated (bs-1519R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin embedded mouse intestine labeled with Rabbit Anti E cadherin/CD324 Polyclonal Antibody, Unconjugated (bs-1519R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin embedded human gastric carcinoma labeled with Anti-pan Cadherin Polyclonal Antibody, Unconjugated (bs-1519R) at 1:200 followed by conjugation to the secondary antibody and DAB staining\n
Image provided by the Independent Validation badge 29742.\n\nHistogram of MCF7 cells stained with Rabbit Anti-E-Cadherin (orange) (bs-1519R, 1:100), isotype control antibody (green), secondary antibody only (blue) and unstained (red).
Lane 1: MCF-7 cell lysates; Lane 2: A431 cell lysates; Lane 3: HepG2 cell lysates probed with SIRT4 Polyclonal Antibody, Unconjugated (bs-1519R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Tissue/cell: A431 cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37°C for 20 min; Antibody incubation with (E cadherin) polyclonal Antibody, Unconjugated (bs-1519R) 1:100, 90 minutes at 37°C; followed by a FITC conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.
Lane 1: Human MDA-MB-231 cell lysates; Lane 2: Human A431 cell lysates; Lane 3: Human A549 cell lysates; Lane 4: Human HepG2 cell lysates probed with E Cadherin Polyclonal Antibody, Unconjugated (bs-1519R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.