bs-1519R [Primary Antibody]
pan Cadherin Polyclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: pan Cadherin

Immunogen Range: 841-882/882


Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 999

Swiss Prot: P12830

Source: KLH conjugated synthetic peptide derived from human E-cadherin

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Background:

Cadherins are calcium-dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types. CDH1 is involved in mechanisms regulating cell-cell adhesions, mobility and proliferation of epithelial cells. Has a potent invasive suppressor role. It is a ligand for integrin alpha-E/beta-7. E-Cad/CTF2 promotes non-amyloidogenic degradation of Abeta precursors. Has a strong inhibitory effect on APP C99 and C83 production.

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)
ELISA(1:500-1000)
FCM(1:20-100)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)
ICC(1:100-500)

Predicted Molecular Weight: 90/97


Cross Reactive Species: Human
Mouse

Predicted Cross Reactive Species: Rat
Dog
Cow
Pig
Horse
Chicken

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Yu, Yang, et al. "RNA interference-mediated knockdown of Notch-1 inhibits migration and invasion, down-regulates matrix metalloproteinases and suppresses NF-κB signaling pathway in trophoblast cells." Acta Histochemica (2014).Read more>>
  • Fan, Hai-Xia, et al. "Sonic hedgehog signaling may promote invasion and metastasis of oral squamous cell carcinoma by activating MMP-9 and E-cadherin expression." Medical Oncology 31.7 (2014): 1-8.Read more>>
  • Wang et al. Expression and clinical significance of autophagic protein LC3B and EMT markers in gastric cancer. (2018) Cancer.Manag.Res. 10:1479-1486Read more>>
  • Liu SQ et al. Sphingosine kinase 1 promotes the metastasis of colorectal cancer by inducing the epithelial‑mesenchymal transition mediated by the FAK/AKT/MMPs axis.Int. J. Oncol. 2018 Oct 25Read more>>
  • Wu Y et al. MFAP5 promotes basal-like breast cancer progression by activating the EMT program.Cell Biosci. 2019 Mar 7;9:24. Read more>>
  • Qu S et al. Effect of Perillaldehyde on Prophylaxis and Treatment of Vaginal Candidiasis in a Murine Model. Front Microbiol. 2019 Jul 2;10:1466.Read more>>
  • Junmin Li. et al. Circ_ZFR contributes to the paclitaxel resistance and progression of non-small cell lung cancer by upregulating KPNA4 through sponging miR-195-5p. Cancer Cell Int. 2021 Dec;21(1):1-15Read more>>
  • Qinhong Xu. et al. Overexpression of circ_0001445 decelerates hepatocellular carcinoma progression by regulating miR-942-5p/ALX4 axis. Biotechnol Lett. 2020 Dec;42(12):2735-2747Read more>>
  • Huajun Wang. et al. LncRNA NEAT1 promotes proliferation, migration, invasion and epithelial-mesenchymal transition process in TGF-2-stimulated lens epithelial cells through regulating the miR-486-5p/SMAD4 axis. Cancer Cell Int. 2020 Dec;20(1):1-12Read more>>
  • Jinzhu Han. et al. Circ_0027599 elevates RUNX1 expression via sponging miR-21-5p on gastric cancer progression. 2021 May 25Read more>>
  • Zhipeng Jiang. et al. EIF4A3-induced circ_0084615 contributes to the progression of colorectal cancer via miR-599/ONECUT2 pathway. J Exp Clin Canc Res. 2021 Dec;40(1):1-15Read more>>
  • Pei Dai. et al. Gimap5 Inhibits Lung Cancer Growth by Interacting With M6PR. Front Oncol. 2021; 11: 699847Read more>>
  • Zheng Wu. et al. FOXD3 suppresses epithelialCmesenchymal transition through direct transcriptional promotion of SMAD7 in esophageal squamous cell carcinoma. 2021 Sep 22Read more>>
  • Zhu, Ting. et al. Irisin/FNDC5 inhibits the epithelialmesenchymal transition of epithelial ovarian cancer cells via the PI3K/Akt pathway. Arch Gynecol Obstet. 2022 Feb;:1-1Read more>>
  • Yang Lili. et al. Elucidating the Novel Mechanism of Ligustrazine in Preventing Postoperative Peritoneal Adhesion Formation. Oxid Med Cell Longev. 2022;2022:9226022Read more>>
  • Zhou Z. et al. Vanillin Derivatives Reverse <i>Fusobacterium nucleatum</i>-Induced Proliferation and Migration of Colorectal Cancer Through E-Cadherin/_-Catenin Pathway.. Front Pharmacol. 2022 Mar;13:841918-841918Read more>>
  • Yue, Huan. et al. LINC02154 promotes the proliferation and metastasis of hepatocellular carcinoma by enhancing SPC24 promoter activity and activating the PI3K-AKT signaling pathway. CELL ONCOL. 2022 May;:1-16Read more>>
  • Qi Rao. et al. Effects of dihydroartemisinin combined with cisplatin on proliferation, apoptosis and migration of HepG2 cells. ONCOL LETT. 2022 Aug;24(2):1-9Read more>>
  • Zheng, Bingxin. et al. Siglec-15-induced autophagy promotes invasion and metastasis of human osteosarcoma cells by activating the epithelialmesenchymal transition and Beclin-1/ATG14 pathway. CELL BIOSCI. 2022 Dec;12(1):1-15Read more>>
  • Yang Liu. et al. Role of Autotaxin in High Glucose-Induced Human ARPE-19 Cells. INT J MOL SCI. 2022 Jan;23(16):9181Read more>>
  • Dr. Jing Tian. et al. Downregulation of SOX21-AS1 alleviated cisplatin resistance in cervical cancer through Epithelial-messenchymal transition (EMT) inhibition. REJUV RES. 2022 Aug 3Read more>>
  • Yu-jie Lu. et al. Ligustilide attenuates airway remodeling in COPD mice by covalently binding to MH2 domain of Smad3 in pulmonary epithelium, disrupting the Smad3-SARA interaction. PHYTOTHER RES. 2022 OcRead more>>
  • Yunhao Ma. et al. CFNC, a neocryptolepine derivative, inhibited the growth of gastric cancer AGS cells by inhibiting PI3K/AKT signaling pathway. EUR J PHARMACOL. 2023 Jan;938:175408Read more>>
  • Zhongjie Tang. et al. Overcoming the On-Target Toxicity in Antibody-Mediated Therapies via an Indirect Active Targeting Strategy. Advanced Science. 2023 Jan;:2206912Read more>>
  • Yingmin Liu. et al. LOXL2 promotes tumor proliferation and metastasis by FAK/Src signaling in esophageal squamous cell carcinoma. ELECTRON J BIOTECHN. 2023 ApRead more>>
  • Hao Cui. et al. Mena as a key enhancer factor of EMT to promote metastasis of human tongue squamous cell carcinoma. ORAL DIS. 2023 MayRead more>>
  • Wu Kunzhe. et al. Co-delivery of curcumin and si-STAT3 with a bioinspired tumor homing for polydopamine nanoparticles for synergistic osteosarcoma therapy. CANCER NANOTECHNOL. 2023 Dec;14(1):1-21Read more>>
  • Jing Liu. et al. Exosomes Promote Atherosclerosis Progression by Regulating Circ_100696/miR-503-5p/PAPPA Axis-Mediated Vascular Smooth Muscle Cells Proliferation and Migration. INT HEART J. 2023 Sep;64(5):918-927Read more>>
  • Yang Xi. et al. circ0005027 Acting as a ceRNA Affects the Malignant Biological Behavior of Hypopharyngeal Squamous Cell Carcinoma by Modulating miR-548c-3p/CDH1 Axis. BIOCHEM GENET. 2023 Nov;:1-16Read more>>
VALIDATION IMAGES

Formalin-fixed and paraffin embedded mouse kidney labeled with Rabbit Anti E cadherin/CD324 Polyclonal Antibody, Unconjugated (bs-1519R) at 1:200 followed by conjugation to the secondary antibody and DAB staining


Formalin-fixed and paraffin embedded mouse intestine labeled with Rabbit Anti E cadherin/CD324 Polyclonal Antibody, Unconjugated (bs-1519R) at 1:200 followed by conjugation to the secondary antibody and DAB staining


Formalin-fixed and paraffin embedded human gastric carcinoma labeled with Anti-pan Cadherin Polyclonal Antibody, Unconjugated (bs-1519R) at 1:200 followed by conjugation to the secondary antibody and DAB staining\n


Image provided by the Independent Validation badge 29742.\n\nHistogram of MCF7 cells stained with Rabbit Anti-E-Cadherin (orange) (bs-1519R, 1:100), isotype control antibody (green), secondary antibody only (blue) and unstained (red).


Lane 1: MCF-7 cell lysates; Lane 2: A431 cell lysates; Lane 3: HepG2 cell lysates probed with SIRT4 Polyclonal Antibody, Unconjugated (bs-1519R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.


Tissue/cell: A431 cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37°C for 20 min; Antibody incubation with (E cadherin) polyclonal Antibody, Unconjugated (bs-1519R) 1:100, 90 minutes at 37°C; followed by a FITC conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.


Lane 1: Human MDA-MB-231 cell lysates; Lane 2: Human A431 cell lysates; Lane 3: Human A549 cell lysates; Lane 4: Human HepG2 cell lysates probed with E Cadherin Polyclonal Antibody, Unconjugated (bs-1519R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.