bs-1522R
[Primary Antibody]
ERK1 (T203/Y205) + ERK2 (T183/Y185) Polyclonal Antibody
DATASHEET
Host: Rabbit
Target Protein: ERK1 (Thr203/Tyr205) + ERK2 (Thr183/Tyr185)
Modification Site: Thr183/Tyr 185
Clonality: Polyclonal
Isotype: IgG
Entrez Gene: 26413
Swiss Prot: P63085
Source: KLH conjugated synthetic phosphopeptide derived from mouse ERK2 around the phosphorylation site of Thr183 and Tyr 185
Purification: Purified by Protein A.
Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Background:
Acts as a transcriptional repressor. Binds to a [GC]AAA[GC] consensus sequence. Repress the expression of interferon gamma-induced genes. Seems to bind to the promoter of CCL5, DMP1, IFIH1, IFITM1, IRF7, IRF9, LAMP3, OAS1, OAS2, OAS3 and STAT1. Transcriptional activity is independent of kinase activity (By similarity).
Size: 100ul
Concentration: 1ug/ul
Applications:
WB(1:300-5000)
ELISA(1:500-1000)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)
Predicted Molecular Weight: 42/44
Cross Reactive Species:
Human
Mouse
Rat
Predicted Cross Reactive Species:
Dog
Cow
Pig
Horse
Chicken
Rabbit
Guinea Pig
For research use only. Not intended for diagnostic or therapeutic use.
PRODUCT SPECIFIC PUBLICATIONS
- Zhao, Hongyu, et al. "Betulin attenuates lung and liver injuries in sepsis."International Immunopharmacology 30 (2016): 50-56.Read more>>
- Cong, Lin, and Wenting Chen. "Neuroprotective Effect of Ginsenoside Rd on Spinal Cord Injury Rats." Basic & Clinical Pharmacology & Toxicology(2016).Read more>>
- Zhao, Haiyan, et al. "Inhibition of endocan attenuates monocrotaline-induced connective tissue disease related pulmonary arterial hypertension." International Immunopharmacology 42 (2017): 115-121.Read more>>
- Yu, Haijie, et al. "Gypenoside Protects Cardiomyocytes against Ischemia-Reperfusion Injury via the Inhibition of Mitogen-Activated Protein Kinase Mediated Nuclear Factor Kappa B Pathway In Vitro and In Vivo." Frontiers in Pharmacology 7 (2016).Read more>>
- Li, Jing, et al. "Downregulation of BRAF‑activated non‑coding RNA suppresses the proliferation, migration and invasion, and induces apoptosis of hepatocellular carcinoma cells." Oncology Letters 14.4 (2017): 4751-4757.Read more>>
- Liu, Xinwei, et al. "Inhibition of BTK protects lungs from trauma-hemorrhagic shock-induced injury in rats." Molecular Medicine Reports 16.1 (2017): 192-200.Read more>>
- Xu et al. RKTG overexpression inhibits proliferation and induces apoptosis of human leukemia cells via suppression of the ERK and PI3K/AKT signaling pathways. (2017) Oncol.Let. 14:965-970Read more>>
- Zhao et al. Adipose differentiation‑related protein knockdown inhibits vascular smooth muscle cell proliferation and migration and attenuates neointima formation. (2017) Mol.Med.Re. 16:3079-3086Read more>>
- Li et al. Downregulation of BRAF-activated non-coding RNA suppresses the proliferation, migration and invasion, and induces apoptosis of hepatocellular carcinoma cells. (2017) Oncol.Lett. 14:4751-4757Read more>>
- Cong and Chen Neuroprotective Effect of Ginsenoside Rd on Spinal Cord Injury Rats. (2016) Basic.Clin.Pharmacol.Toxicol. Read more>>
- Lin X et al. TFF3 Contributes to Epithelial-Mesenchymal Transition (EMT) in Papillary Thyroid Carcinoma Cells via the MAPK/ERK Signaling Pathway.(2018)J Cancer;Oct 31;9(23):4430-4439. Read more>>
- Wang G et al. Protective Effect of Methane-Rich Saline on Acetic Acid-Induced Ulcerative Colitis via Blockingthe TLR4/NF-κB/MAPK Pathway and Promoting IL-10/JAK1/STAT3-Mediated Anti-inflammatory Response. Oxid Med Cell Longev. 2019 Apr 28;2019:7850324.Read more>>
- Pan LX et al. TMEM100 mediates inflammatory cytokines secretion in hepatic stellate cells and its mechanism research. Toxicol Lett. 2019 Jan 10. pii: S0378-4274(18)31704-1. Read more>>
- Zhou J et al. Paeonol antagonizes oncogenesis of osteosarcoma by inhibiting the function of TLR4/MAPK/NF-κB pathway. Acta Histochem. 2019 Oct 3:151455. Read more>>
- Zhou C et al. Combining transcatheter arterial embolization with iodized oil containing Apatinib inhibits HCC growth and metastasis. Sci Rep. 2020 Feb 19;10(1):2964. Read more>>
- Zhou C et al. Combining transcatheter arterial embolization with iodized oil containing Apatinib inhibits HCC growth and metastasis. Sci Rep. 2020 Feb 19;10(1):2964. Read more>>
- Wang L et al. 1\(4\((5\chloro\4\((2\(isopropylsulfonyl)phenyl)amino)pyrimidin\2\yl)amino)\3\methoxyphenyl)\3\(2\(dimethylamino)ethyl)imidazolidin\2\one (ZX\42), a novel ALK inhibitor, induces apoptosis and protective autophagy in H2228 cells. J Pharm Pharmacol. 2020 Oct;72(10):1370-1382.Read more>>
- Lei Yuet al. TMF, a natural dihydroflavonoid isolated from Scutellaria javanica Jungh, stimulates anticancer activity of s180 cancer-bearing mice, induces apoptosis, inhibits invasion and migration on HepG-2?cells. J Ethnopharmacol. 2020 Dec 5;263:113072.Read more>>
- Le Zhuang. et al. Evaluation of the effects of IL?22 on the proliferation and differentiation of keratinocytes in?vitro. Mol Med Rep. 2020 Oct;22(4):2715-2722Read more>>
- Fang Jia. et al. Cytotoxicity and anti-inflammatory effect of a novel diminazene aceturate derivative in bovine mammary epithelial cells. Res Vet Sci. 2021 Jul;137:102Read more>>
- Fengyan Huang. et al. SLC34A2 Up-regulation And SLC4A4 Down-regulation Correlates With Invasion, Metastasis, And The MAPK Signaling Pathway In Papillary Thyroid Carcinomas. J Cancer. 2021; 12(18): 5439C5453Read more>>
- Junfeng Ke. et al. CTI-2 Inhibits Metastasis and Epithelial-Mesenchymal Transition of Breast Cancer Cells by Modulating MAPK Signaling Pathway. Int J Mol Sci. 2021 Jan;22(22):12229Read more>>
- Meiqiong Wu. et al. Suppression of NADPH oxidase 4 inhibits PM2.5-induced cardiac fibrosis through ROS-P38 MAPK pathway. SCI TOTAL ENVIRON. 2022 Apr;:155558Read more>>
- Zhang, Di. et al. Salvia miltiorrhiza polysaccharides ameliorates Staphylococcus aureus-induced mastitis in rats by inhibiting activation of the NF-_B and MAPK signaling pathways. BMC VET RES. 2022 Dec;18(1):1-11Read more>>
- Shuting Wei. et al. Particle matters induce airway epithelial barrier dysfunction in vivo and in vitro: from a more realistic inhalation scenario. ENVIRON SCI-NANO. 2022 JuRead more>>
- Wen-Bo Zhang. et al. Down-regulating of MFN2 promotes vascular calcification via regulating RAS-RAF-ERK1/2 pathway. INT J CARDIOL. 2022 JuRead more>>
- Zhang Xiangjun. et al. DAD3 targets ACE2 to inhibit the MAPK and NF-B signalling pathways and protect against LPS-induced inflammation in bovine mammary epithelial cells. VET RES. 2022 Dec;53(1):1-13Read more>>
- Huawei Liu. et al. Integrated multi-omics reveals the beneficial role of chlorogenic acid in improving the growth performance and immune function of immunologically-stressed broilers. ANIM NUTR. 2023 MayRead more>>
- Lemiao Zhong. et al. Mycoplasma synoviae induce spleen tissue damage and inflammatory response of chicken through oxidative stress and apoptosis. VIRULENCE. 2023 Nov 16Read more>>
- Xinyun Qin. et al. Regulation of the intestinal flora using polysaccharides from Callicarpa nudiflora Hook to alleviate ulcerative colitis and the molecular mechanisms involved. INT J BIOL MACROMOL. 2024 Feb;258:128887Read more>>
- Keyi Nong. et al. Effect of the Pseudopleuronectes americanus-derived Pleurocidin on DSS-induced Ulcerative colitis in mice and its preliminary molecular mechanisms. INT IMMUNOPHARMACOL. 2024 Mar;130:111757Read more>>
VALIDATION IMAGES
L1 rat liver lysates L2 rat kidney lysates probed with Rabbit Anti-ERK1 (Thr203/Tyr205) + ERK2 (Thr183/Tyr185) Polyclonal Antibody, Unconjugated (bs-1522R) at 1:200 overnight at 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 for 90 min at RT. Predicted band 42kD. Observed band size: 42kD
Formalin-fixed and paraffin embedded mouse embryo tissue labeled with Rabbit Anti-ERK1 (Thr203/Tyr205) + ERK2 (Thr183/Tyr185) Polyclonal Antibody, Unconjugated (bs-1522R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Tissue/cell: Hela cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37°C for 20 min; Antibody incubation with (phospho-ERK1 + 2 (Thr183/Tyr185)) polyclonal Antibody, Unconjugated (bs-1522R) 1:100, 90 minutes at 37°C; followed by a FITC conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.
Tissue/cell: HUVEC cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37°C for 20 min; Antibody incubation with (phospho-ERK1 + 2 (Thr183/Tyr185)) Polyclonal Antibody, Unconjugated (bs-1522R) 1:100, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody (bs-0295G-FITC) at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.