bs-1524R
[Primary Antibody]
GlyR alpha 1/2/3 Polyclonal Antibody
www.biossusa.com
support@biossusa.com
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
support@biossusa.com
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET
Host: Rabbit
Target Protein: GlyR alpha 1/2/3
Clonality: Polyclonal
Isotype: IgG
Source: KLH conjugated synthetic peptide derived from human GlyR alpha 1
Purification: Purified by Protein A.
Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage: Store at -20°C for 12 months.
Background:
Glycine receptors are members of the ligand-gated ion channel superfamily, which mediate fast inhibitory neurotransmission. The receptors are pentameric membrane proteins which form chloride channels. Binding of glycine to its receptor produces an increase in chloride conductance and membrane hyperpolarisation. Four genes encoding glycine receptor alpha subunits have been identified, together with a single beta polypeptide. Each subunit consists of a large extracellular N-terminal region, four transmembrane domains, and a large cytoplasmic domain.
Size: 100µL
Concentration: 1ug/ul
Applications:
WB(1:300-5000)
IHC-P(1:200-400)
IF(IHC-P)(1:50-200)
Cross Reactive Species:
Human
Mouse
Rat
For research use only. Not intended for diagnostic or therapeutic use.
VALIDATION IMAGES
Lane 1: mouse brain lysates Lane 2: mouse embryo lysates probed with Anti GlyR alpha 1/2/3 Polyclonal Antibody, Unconjugated (bs-1524R) at 1:200 in 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 90min in RT. Predicted band 49kD. Observed band size: 49kD.
Lane 1: Mouse Cerebrum tissue lysates; Lane 2: Mouse Cerebellum tissue lysates; Lane 3: Mouse Testis tissue lysates; Lane 4: Mouse Lung tissue lysates; Lane 5: Rat Cerebrum tissue lysates; Lane 6: Rat Cerebellum tissue lysates; Lane 7: Rat Testis tissue lysates; Lane 8: Human HeLa cell lysates; Lane 9: Human A431 cell lysates; Lane 10: Human SH-SY5Y cell lysates probed with GlyR alpha 1 + 2 + 3 Polyclonal Antibody, Unconjugated (bs-1524R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at room temperature for 60 min.