bs-1529R [Primary Antibody]
CD46/MCP Polyclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: CD46/MCP

Immunogen Range: 251-355/355


Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 4179

Swiss Prot: P15529

Source: KLH conjugated synthetic peptide derived from Mouse CD46

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Background:

Acts as a cofactor for complement factor I, a serine protease which protects autologous cells against complement-mediated injury by cleaving C3b and C4b deposited on host tissue. May be involved in the fusion of the spermatozoa with the oocyte during fertilization. Also acts as a costimulatory factor for T-cells which induces the differentiation of CD4+ into T-regulatory 1 cells. T-regulatory 1 cells suppress immune responses by secreting interleukin-10, and therefore are thought to prevent autoimmunity. A number of viral and bacterial pathogens seem to exploit this property and directly induce an immunosuppressive phenotype in T-cells by binding to CD46.

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)
ELISA(1:500-1000)
FCM(1:20-100)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)
ICC(1:100-500)

Predicted Molecular Weight: 43


Cross Reactive Species: Human
Mouse

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

L1 mouse brain lysates L2 human colon carcinoma lysates probed with Anti CD46/MCP Polyclonal Antibody, Unconjugated (bs-1529R) at 1:200 in 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 90min in RT. Predicted band 43kD. Observed band size: 43kD


Paraformaldehyde-fixed, paraffin embedded Human colon cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with CD46/MCP Polyclonal Antibody, Unconjugated (bs-1529R) at 1:400 overnight at 4°C, DAB staining.


Lane 1: Mouse Testis lysates probed with CD46/MCP Polyclonal Antibody, Unconjugated (bs-1529R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.


Lane 1: Mouse Epididymis lysates probed with CD46/MCP Polyclonal Antibody, Unconjugated (bs-1529R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.


Lane 1: Mouse Placenta tissue lysates; Lane 2: Mouse Lung tissue lysates; Lane 3: Human K562 cell lysates; Lane 4: Human HeLa cell lysates; Lane 5: Human MOLT4 cell lysates; Lane 6: Human MCF-7 cell lysates; Lane 7: Human Daudi cell lysates probed with CD46 Polyclonal Antibody, Unconjugated (bs-1529R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at room temperature for 60 min.