bs-1531R
[Primary Antibody]
ATF4/CREB-2 Polyclonal Antibody
DATASHEET
Host: Rabbit
Target Protein: ATF4/CREB-2
Immunogen Range: 251-351/351
Clonality: Polyclonal
Isotype: IgG
Entrez Gene: 468
Swiss Prot: P18848
Source: KLH conjugated synthetic peptide derived from human ATF4
Purification: Purified by Protein A.
Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Background:
Transcriptional activator. Binds the cAMP response element (CRE) (consensus: 5'-GTGACGT[AC][AG]-3'), a sequence present in many viral and cellular promoters. Cooperates with FOXO1 in osteoblasts to regulate glucose homeostasis through suppression of beta-cell production and decrease in insulin production (By similarity). It binds to a Tax-responsive enhancer element in the long terminal repeat of HTLV-I. Regulates the induction of DDIT3/CHOP and asparagine synthetase (ASNS) in response to ER stress. In concert with DDIT3/CHOP, activates the transcription of TRIB3 and promotes ER stress-induced neuronal apoptosis by regulating the transcriptional induction of BBC3/PUMA. Activates transcription of SIRT4. Regulates the circadian expression of the core clock component PER2 and the serotonin transporter SLC6A4. Binds in a circadian time-dependent manner to the cAMP response elements (CRE) in the SLC6A4 and PER2 promoters and periodically activates the transcription of these genes.
Size: 100ul
Concentration: 1ug/ul
Applications:
WB(1:300-5000)
ELISA(1:500-1000)
FCM(1:20-100)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)
Predicted Molecular Weight: 38
Cross Reactive Species:
Human
Mouse
Rat
Dog
Rabbit
Predicted Cross Reactive Species:
Cow
Sheep
Pig
Horse
For research use only. Not intended for diagnostic or therapeutic use.
PRODUCT SPECIFIC PUBLICATIONS
- Jiang, H-Q., et al. "Guanabenz Delays the Onset of Disease Symptoms, Extends Lifespan, Improves Motor Performance and Attenuates Motor Neuron Loss in the SOD1 G93A Mouse Model of Amyotrophic Lateral Sclerosis." Neuroscience (2014).Read more>>
- Jiang, Mingfang, et al. "Down-regulation of miR-384-5p Attenuates Rotenone-induced Neurotoxicity in Dopaminergic SH-SY5Y Cells Through Inhibiting Endoplasmic Reticulum Stress." American Journal of Physiology-Cell Physiology (2016): ajpcell-00226.Read more>>
- Hayashi, Keitaro, et al. "HOXB9 acts as a negative regulator of activated human T cells in response to amino acid deficiency." Immunology and Cell Biology (2016).Read more>>
- Yan et al. Cytotoxicity of CdTe quantum dots in human umbilical vein endothelial cells: the involvement of cellular uptake and induction of pro-apoptotic endoplasmic reticulum stress. (2016) Int.J.Nanomedicine. 11:529-42Read more>>
- Li,et al.Silver nanoparticles induce SH-SY5Y cell apoptosis via endoplasmic reticulum- and mitochondrial pathways that lengthen endoplasmic reticulum-mitochondria contact sites and alter inositol-3-phosphate receptor function.(2018) Toxicology Letters. 285:156-167.Read more>>
- Zhang,et al.Ectopic expression of Miro 1 ameliorates seizure and inhibits hippocampal neurodegeneration in a mouse pilocarpine epilepsy model.(2018) Biochemistry and Cell Biology. :.Read more>>
- Wang,et al.Development of a SPR aptasensor containing oriented aptamer for direct capture and detection of tetracycline in multiple honey samples.(2018) Biosensors & Bioelectronics. 109:1-7.Read more>>
- Shen C et al. In vitro culture expansion impairs chondrogenic differentiation and the therapeutic effect of mesenchymal stem cells by regulating the unfolded protein response.(2018)J Biol Eng. Nov 20;12:26. Read more>>
- Fang et al. Melatonin prevents senescence of canine adipose-derived mesenchymal stem cells through activating NRF2 and inhibiting ER stress. (2018) Aging.(Albany.NY). 10:2954-2974Read more>>
- Wang L et al. Radioprotective effect of Hohenbuehelia serotina polysaccharides through mediation of ER apoptosis pathway in vivo. Int J Biol Macromol. 2019 Apr 15;127:18-26. Read more>>
- Hou Y et al. Longzhibu disease and its therapeutic effects by traditional Tibetan medicine: Ershi-wei Chenxiang pills. J Ethnopharmacol. 2019 Nov 24:112426. Read more>>
- ingwei Yang et al. Polychlorinated Biphenyl Quinone Promotes Atherosclerosis through Lipid Accumulation and Endoplasmic Reticulum Stress via CD36. Chem Res Toxicol. 2020 Jun 15;33(6):1497-1507.Read more>>
- Yuting Wang. et al. Polybrominated diphenyl ethers quinone-induced intracellular protein oxidative damage triggers ubiquitin-proteasome and autophagy-lysosomal system activation in LO2 cells. Chemosphere. 2021 Jul;275:130034Read more>>
- Luo Liwen. et al. Cartilage Endplate Stem Cells Transdifferentiate Into Nucleus Pulposus Cells via Autocrine Exosomes. Front Cell Dev Biol. 2021 Mar;9:482Read more>>
- Wiebke Sachs. et al. Distinct Modes of Balancing Glomerular Cell Proteostasis in Mucolipidosis Type II and III Prevent Proteinuria. J Am Soc Nephrol. 2020 Aug;31(8):1796-1814Read more>>
- Chunyue Wang. et al. Neuroprotective effects of verbascoside against Alzheimers disease via the relief of endoplasmic reticulum stress in A-exposed U251 cells and APP/PS1 mice. J Neuroinflamm. 2020 Dec;17(1):1-16Read more>>
- Xiao-meng Wei. et al. Endoplasmic Reticulum Stress-Activated PERK-eIF2-ATF4 Signaling Pathway is Involved in the Ameliorative Effects of Ginseng Polysaccharides against Cisplatin-Induced Nephrotoxicity in Mice. Acs Omega. 2021;XXXX(XXX):XXX-XXXRead more>>
- Hsin-Ping Pao. et al. Suppression of Endoplasmic Reticulum Stress by 4-PBA Protects Against Hyperoxia-Induced Acute Lung Injury via Up-Regulating Claudin-4 Expression. Front Immunol. 2021; 12: 674316Read more>>
- Junxiong Wang. et al. Protective Effects of Taraxasterol against Deoxynivalenol-Induced Damage to Bovine Mammary Epithelial Cells. Toxins. 2022 Mar;14(3):211Read more>>
- Jian Chen. et al. Astragalus polysaccharide alleviates transport stress-induced heart injury in newly hatched chicks via ERS-UPR-Autophagy dependent pathway. POULTRY SCIENCE. 2022 Jun;:10203Read more>>
- Yurong Fu. et al. Zearalenone Promotes LPS-Induced Oxidative Stress, Endoplasmic Reticulum Stress, and Accelerates Bovine Mammary Epithelial Cell Apoptosis. INT J MOL SCI. 2022 Jan;23(18):10925Read more>>
- Kunlin Wu. et al. Histone deacetylase inhibitor panobinostat in combination with rapamycin confers enhanced efficacy against triple-negative breast cancer. EXP CELL RES. 2022 Sep;:113362Read more>>
- Ming-Zhi Huang. et al. Exosomes from artesunate-treated bone marrow-derived mesenchymal stem cells transferring SNHG7 to promote osteogenesis via TAF15-RUNX2 pathway. REGEN MED. 2022 Oct 02Read more>>
- Yin, Junhao. et al. CYP51-mediated cholesterol biosynthesis is required for the proliferation of CD4+ T cells in Sjogrens syndrome. CLIN EXP MED. 2022 Nov;:1-21Read more>>
- Yunjung Lee. et al. Synergistic effect of n-3 PUFA and probiotic supplementation on bone loss induced by chronic mild stress through the brainCgutCbone axis. J FUNCT FOODS. 2023 Jan;100:105363Read more>>
- Yu Li. et al. Activating transcription factor 4 drives the progression of diabetic cardiac fibrosis. ESC HEART FAIL. 2023 JuRead more>>
- Dawei Song. et al. Effect and mechanism of fluctuant glucose on restraining implant osseointegration in diabetes. ORAL DIS. 2023 JuRead more>>
- Wei Yu. et al. Glucose promotes cell growth and casein synthesis via ATF4/Nrf2-Sestrin2- AMPK-mTORC1 pathway in dairy cow mammary epithelial cells. ANIM BIOTECHNOL. 2023 Jul 12Read more>>
- Shulan Yin. et al. Chitosan oligosaccharide attenuates acute kidney injury and renal interstitial fibrosis induced by ischemia-reperfusion. RENAL FAILURE. 2023;45(1):Article: 2238831Read more>>
- Shiokawa Daisuke. et al. Elevated stress response marks deeply quiescent reserve cells of gastric chief cells. COMMUN BIOL. 2023 Nov;6(1):1-1Read more>>
VALIDATION IMAGES
Lane 1:Mouse embryo lysate Lane 2:Mouse eye lysate probed with Rabbit Anti-ATF4/CREB-2 Polyclonal Antibody, Unconjugated (bs-1531R) at 1:300 overnight at 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:5000 for 90 min at 37˚C.
Formalin-fixed and paraffin embedded mouse brain labeled with Anti-ATF4/CREB-2 Polyclonal Antibody, Unconjugated (bs-1531R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin embedded human rectal carcinoma labeled with Anti-ATF4/CREB-2 Polyclonal Antibody, Unconjugated (bs-1531R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Paraformaldehyde-fixed, paraffin embedded Rat spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with ATF4/CREB-2 Polyclonal Antibody, Unconjugated (bs-1531R) at 1:200 overnight at 4°C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Mouse brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with ATF4/CREB-2 Polyclonal Antibody, Unconjugated (bs-1531R) at 1:200 overnight at 4°C, DAB staining.
Lane 1: Rat Cerebrum lysates; Lane 2: Mouse Cerebrum lysates probed with ATF4/CREB-2 Polyclonal Antibody, Unconjugated (bs-1531R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Paraformaldehyde-fixed, paraffin embedded Rat lung; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with ATF4/CREB-2 Polyclonal Antibody, Unconjugated (bs-1531R) at 1:200 overnight at 4°C, DAB staining.
Mouse spleen cells were fixed with 4% PFA for 10min at room temperature,permeabilized with 90% ice-cold methanol for 20 min at -20℃, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with ATF4 Antibody(bs-1531R)at 1:50 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).
Paraformaldehyde-fixed, paraffin embedded Rat brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with ATF4/CREB-2 Polyclonal Antibody, Unconjugated (bs-1531R) at 1:200 overnight at 4°C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Human brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with ATF4/CREB-2 Polyclonal Antibody, Unconjugated (bs-1531R) at 1:200 overnight at 4°C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Human breast cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with ATF4/CREB-2 Polyclonal Antibody, Unconjugated (bs-1531R) at 1:200 overnight at 4°C, DAB staining.
Hela cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37°C for 20 min; Antibody incubation with (ATF4) polyclonal Antibody, Unconjugated (bs-1531R) 1:100, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.
Lane 1: Mouse Spleen Lysates; Lane 2: Mouse Lung Lysates; Lane 3: Mouse Brain Lysates; Lane 4: Rat Brain Lysates; Lane 5: Human Hela cell Lysates; Lane 6: Human HepG2 cell Lysates. Probed with ATF4 polyclonal Antibody, unconjugated (bs-1531R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
U2OS cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37°C for 20 min; Antibody incubation with (ATF4) polyclonal Antibody, Unconjugated (bs-1531R) 1:100, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.
Paraformaldehyde-fixed, paraffin embedded (rat skin); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (ATF4/CREB-2) Polyclonal Antibody, Unconjugated (bs-1531R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (mouse skin); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (ATF4/CREB-2) Polyclonal Antibody, Unconjugated (bs-1531R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (Human skin cancer); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (ATF4/CREB-2) Polyclonal Antibody, Unconjugated (bs-1531R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (Human breast cancer); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (ATF4/CREB-2) Polyclonal Antibody, Unconjugated (bs-1531R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (Human brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (ATF4/CREB-2) Polyclonal Antibody, Unconjugated (bs-1531R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Lane 1: Mouse Cerebrum tissue lysates; Lane 2: Rat Cerebrum tissue lysates; Lane 3: Human HeLa cell lysates; Lane 4: Human Jurkat cell lysates; Lane 5: Human HL-60 cell lysates probed with ATF4 Polyclonal Antibody, Unconjugated (bs-1531R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.