bs-1531R-A647
[Conjugated Primary Antibody]
ATF4/CREB-2 Polyclonal Antibody, ALEXA FLUOR® 647 Conjugated
DATASHEET
Host: Rabbit
Target Protein: ATF4/CREB-2
Immunogen Range: 251-351/351
Clonality: Polyclonal
Isotype: IgG
Entrez Gene: 468
Swiss Prot: P18848
Source: KLH conjugated synthetic peptide derived from human ATF4
Purification: Purified by Protein A.
Storage Buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Background:
Transcriptional activator. Binds the cAMP response element (CRE) (consensus: 5'-GTGACGT[AC][AG]-3'), a sequence present in many viral and cellular promoters. Cooperates with FOXO1 in osteoblasts to regulate glucose homeostasis through suppression of beta-cell production and decrease in insulin production (By similarity). It binds to a Tax-responsive enhancer element in the long terminal repeat of HTLV-I. Regulates the induction of DDIT3/CHOP and asparagine synthetase (ASNS) in response to ER stress. In concert with DDIT3/CHOP, activates the transcription of TRIB3 and promotes ER stress-induced neuronal apoptosis by regulating the transcriptional induction of BBC3/PUMA. Activates transcription of SIRT4. Regulates the circadian expression of the core clock component PER2 and the serotonin transporter SLC6A4. Binds in a circadian time-dependent manner to the cAMP response elements (CRE) in the SLC6A4 and PER2 promoters and periodically activates the transcription of these genes.
Conjugation: ALEXA FLUOR® 647
Excitation/ Emission: 650nm/665nm
Size: 100ul
Concentration: 1ug/ul
Applications:
WB(1:300-5000)
FCM(1:20-100)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)
Predicted Molecular Weight: 38
Cross Reactive Species:
Human
Mouse
Rat
Dog
Rabbit
Predicted Cross Reactive Species:
Cow
Sheep
Pig
Horse
For research use only. Not intended for diagnostic or therapeutic use.
PRODUCT SPECIFIC PUBLICATIONS
- Jiang, H-Q., et al. "Guanabenz Delays the Onset of Disease Symptoms, Extends Lifespan, Improves Motor Performance and Attenuates Motor Neuron Loss in the SOD1 G93A Mouse Model of Amyotrophic Lateral Sclerosis." Neuroscience (2014).Read more>>
- Jiang, Mingfang, et al. "Down-regulation of miR-384-5p Attenuates Rotenone-induced Neurotoxicity in Dopaminergic SH-SY5Y Cells Through Inhibiting Endoplasmic Reticulum Stress." American Journal of Physiology-Cell Physiology (2016): ajpcell-00226.Read more>>
- Hayashi, Keitaro, et al. "HOXB9 acts as a negative regulator of activated human T cells in response to amino acid deficiency." Immunology and Cell Biology (2016).Read more>>
- Yan et al. Cytotoxicity of CdTe quantum dots in human umbilical vein endothelial cells: the involvement of cellular uptake and induction of pro-apoptotic endoplasmic reticulum stress. (2016) Int.J.Nanomedicine. 11:529-42Read more>>
- Li,et al.Silver nanoparticles induce SH-SY5Y cell apoptosis via endoplasmic reticulum- and mitochondrial pathways that lengthen endoplasmic reticulum-mitochondria contact sites and alter inositol-3-phosphate receptor function.(2018) Toxicology Letters. 285:156-167.Read more>>
- Zhang,et al.Ectopic expression of Miro 1 ameliorates seizure and inhibits hippocampal neurodegeneration in a mouse pilocarpine epilepsy model.(2018) Biochemistry and Cell Biology. :.Read more>>
- Wang,et al.Development of a SPR aptasensor containing oriented aptamer for direct capture and detection of tetracycline in multiple honey samples.(2018) Biosensors & Bioelectronics. 109:1-7.Read more>>
- Shen C et al. In vitro culture expansion impairs chondrogenic differentiation and the therapeutic effect of mesenchymal stem cells by regulating the unfolded protein response.(2018)J Biol Eng. Nov 20;12:26. Read more>>
- Fang et al. Melatonin prevents senescence of canine adipose-derived mesenchymal stem cells through activating NRF2 and inhibiting ER stress. (2018) Aging.(Albany.NY). 10:2954-2974Read more>>
- Wang L et al. Radioprotective effect of Hohenbuehelia serotina polysaccharides through mediation of ER apoptosis pathway in vivo. Int J Biol Macromol. 2019 Apr 15;127:18-26. Read more>>
- Hou Y et al. Longzhibu disease and its therapeutic effects by traditional Tibetan medicine: Ershi-wei Chenxiang pills. J Ethnopharmacol. 2019 Nov 24:112426. Read more>>
- ingwei Yang et al. Polychlorinated Biphenyl Quinone Promotes Atherosclerosis through Lipid Accumulation and Endoplasmic Reticulum Stress via CD36. Chem Res Toxicol. 2020 Jun 15;33(6):1497-1507.Read more>>
- Yuting Wang. et al. Polybrominated diphenyl ethers quinone-induced intracellular protein oxidative damage triggers ubiquitin-proteasome and autophagy-lysosomal system activation in LO2 cells. Chemosphere. 2021 Jul;275:130034Read more>>
- Luo Liwen. et al. Cartilage Endplate Stem Cells Transdifferentiate Into Nucleus Pulposus Cells via Autocrine Exosomes. Front Cell Dev Biol. 2021 Mar;9:482Read more>>
- Wiebke Sachs. et al. Distinct Modes of Balancing Glomerular Cell Proteostasis in Mucolipidosis Type II and III Prevent Proteinuria. J Am Soc Nephrol. 2020 Aug;31(8):1796-1814Read more>>
- Chunyue Wang. et al. Neuroprotective effects of verbascoside against Alzheimers disease via the relief of endoplasmic reticulum stress in A-exposed U251 cells and APP/PS1 mice. J Neuroinflamm. 2020 Dec;17(1):1-16Read more>>
- Xiao-meng Wei. et al. Endoplasmic Reticulum Stress-Activated PERK-eIF2-ATF4 Signaling Pathway is Involved in the Ameliorative Effects of Ginseng Polysaccharides against Cisplatin-Induced Nephrotoxicity in Mice. Acs Omega. 2021;XXXX(XXX):XXX-XXXRead more>>
- Hsin-Ping Pao. et al. Suppression of Endoplasmic Reticulum Stress by 4-PBA Protects Against Hyperoxia-Induced Acute Lung Injury via Up-Regulating Claudin-4 Expression. Front Immunol. 2021; 12: 674316Read more>>
- Junxiong Wang. et al. Protective Effects of Taraxasterol against Deoxynivalenol-Induced Damage to Bovine Mammary Epithelial Cells. Toxins. 2022 Mar;14(3):211Read more>>
- Jian Chen. et al. Astragalus polysaccharide alleviates transport stress-induced heart injury in newly hatched chicks via ERS-UPR-Autophagy dependent pathway. POULTRY SCIENCE. 2022 Jun;:10203Read more>>
- Yurong Fu. et al. Zearalenone Promotes LPS-Induced Oxidative Stress, Endoplasmic Reticulum Stress, and Accelerates Bovine Mammary Epithelial Cell Apoptosis. INT J MOL SCI. 2022 Jan;23(18):10925Read more>>
- Kunlin Wu. et al. Histone deacetylase inhibitor panobinostat in combination with rapamycin confers enhanced efficacy against triple-negative breast cancer. EXP CELL RES. 2022 Sep;:113362Read more>>
- Ming-Zhi Huang. et al. Exosomes from artesunate-treated bone marrow-derived mesenchymal stem cells transferring SNHG7 to promote osteogenesis via TAF15-RUNX2 pathway. REGEN MED. 2022 Oct 02Read more>>
- Yin, Junhao. et al. CYP51-mediated cholesterol biosynthesis is required for the proliferation of CD4+ T cells in Sjogrens syndrome. CLIN EXP MED. 2022 Nov;:1-21Read more>>
- Yunjung Lee. et al. Synergistic effect of n-3 PUFA and probiotic supplementation on bone loss induced by chronic mild stress through the brainCgutCbone axis. J FUNCT FOODS. 2023 Jan;100:105363Read more>>
- Yu Li. et al. Activating transcription factor 4 drives the progression of diabetic cardiac fibrosis. ESC HEART FAIL. 2023 JuRead more>>
- Dawei Song. et al. Effect and mechanism of fluctuant glucose on restraining implant osseointegration in diabetes. ORAL DIS. 2023 JuRead more>>
- Wei Yu. et al. Glucose promotes cell growth and casein synthesis via ATF4/Nrf2-Sestrin2- AMPK-mTORC1 pathway in dairy cow mammary epithelial cells. ANIM BIOTECHNOL. 2023 Jul 12Read more>>
- Shulan Yin. et al. Chitosan oligosaccharide attenuates acute kidney injury and renal interstitial fibrosis induced by ischemia-reperfusion. RENAL FAILURE. 2023;45(1):Article: 2238831Read more>>
- Shiokawa Daisuke. et al. Elevated stress response marks deeply quiescent reserve cells of gastric chief cells. COMMUN BIOL. 2023 Nov;6(1):1-1Read more>>
VALIDATION IMAGES