bs-15450R [Primary Antibody]
HELZ Polyclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: HELZ

Immunogen Range: 1-100/1942


Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 9931

Swiss Prot: P42694

Source: KLH conjugated synthetic peptide derived from human HELZ

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Background:

May act as a helicase that plays a role in RNA metabolism in multiple tissues and organs within the developing embryo.

Size: 100ul

Concentration: 1ug/ul

Applications: ELISA(1:500-1000)
FCM(1:20-100)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)
ICC(1:100-500)

Predicted Molecular Weight: 219


Cross Reactive Species: Rat

Predicted Cross Reactive Species: Human
Mouse

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Paraformaldehyde-fixed, paraffin embedded rat brain; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37°C for 20min; Antibody incubation with HELZ Polyclonal Antibody, Unconjugated (bs-15450R ) at 1:500 overnight at 4°C, followed by a conjugated secondary and DAB staining.