Host: Rabbit
Target Protein: HENMT1
Immunogen Range: 1-100/393
Clonality: Polyclonal
Isotype: IgG
Source: KLH conjugated synthetic peptide derived from human HENMT1
Purification: Purified by Protein A.
Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Background:
Size: 100ul
Concentration: 1ug/ul
Applications:
WB(1:300-5000)
ELISA(1:500-1000)
FCM(1:20-100)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)
ICC(1:100-500)
Predicted Molecular Weight: 45
Cross Reactive Species:
Human
Rat
Predicted Cross Reactive Species: Mouse
For research use only. Not intended for diagnostic or therapeutic use.
Paraformaldehyde-fixed, paraffin embedded rat brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with HENMT1 Polyclonal Antibody, Unconjugated (bs-15454R) at 1:500 overnight at 4°C, followed by a conjugated secondary for 20 minutes and DAB staining
Paraformaldehyde-fixed, paraffin embedded human skin; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with HENMT1 Polyclonal Antibody, Unconjugated (bs-15454R) at 1:500 overnight at 4°C, followed by a conjugated secondary for 20 minutes and DAB staining.