bs-15494R-Cy5

DATASHEET

Host: Rabbit

Target Protein: Sumo2

Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 6613

Swiss Prot: P61956

Source: KLH conjugated synthetic peptide derived from human Sumo2

Purification: Purified by Protein A.

Storage Buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Background:

The small ubiquitin-related modifier (SUMO) proteins, which include SUMO-1, SUMO-2 and SUMO-3, belong to the ubiquitin-like protein family. Like ubiquitin, the SUMO proteins are synthesized as precursor proteins that undergo processing before conjugation to target proteins. Also, both utilize the E1, E2, and E3 cascade enzymes for conjugation. However, SUMO and ubiquitin differ with respect to targeting. Ubiquitination predominantly targets proteins for degradation, whereas sumoylation targets proteins to a variety of cellular processing, including nuclear transport, transcriptional regulation, apoptosis and protein stability. The unconjugated SUMO-1, SUMO-2 and SUMO-3 proteins localize to the nuclear membrane, nuclear bodies and cytoplasm, respectively. SUMO-1 utilizes Ubc9 for conjugation to several target proteins, which include IkBa, MDM2, p53, PML and Ran GAP1. SUMO-2 and SUMO-3 contribute to a greater percentage of protein modification than does SUMO-1, and unlike SUMO-1, they can form polymeric chains. In addition, SUMO-3 regulates b-Amyloid generation and may be critical in the onset or progression of Alzheimer’s disease

Conjugation: Cy5

Excitation/ Emission: 625,650nm/670nm

Size: 100µL

Concentration: 1ug/ul

Applications: IF(1:100-500)

Predicted Molecular Weight: 11

Cross Reactive Species: Human
Mouse

Predicted Cross Reactive Species: Rat
Cow
Pig
Rabbit

For research use only. Not intended for diagnostic or therapeutic use.