bs-1554R [Primary Antibody]
AQP5 Polyclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: Aquaporin 5

Immunogen Range: 201-265/265


Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 11830

Swiss Prot: Q9WTY4

Source: KLH conjugated synthetic peptide derived from mouse AQP5

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Background:

Forms a water-specific channel. Implicated in the generation of saliva, tears, and pulmonary secretions.

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)
ELISA(1:500-1000)
FCM(1:20-100)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)

Predicted Molecular Weight: 29


Cross Reactive Species: Human
Mouse
Rat

Predicted Cross Reactive Species: Dog
Cow
Sheep
Pig
Horse

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Lim, Jae-Yol, et al. "Establishment and Characterization of Mesenchymal Stem Cell-like Clonal Stem Cells from Mouse Salivary Glands." Tissue Engineering (2014).Read more>>
  • Yi, TacGhee, et al. "Single Cell Clones Purified from Human Parotid Glands Display Features of Multipotent Epitheliomesenchymal Stem Cells." Scientific Reports 6 (2016).Read more>>
  • Wei W et al. Estradiol Induced Estrogen Receptor-mediated Transcription and Expression of Aquaporin5.Chemical Research in Chinese Universities,2019 35(2), 239–244.  Read more>>
  • Wang D et al. Suppression of high-mobility group box 1 ameliorates xerostomia in a sjogren syndrome-triggered mouse model. Can J Physiol Pharmacol. 2020 Jan 14. Read more>>
  • Sato K et al. Different Prognostic Implications of Aquaporin-1 and Aquaporin-5 Expression among Different Histological Types of Ovarian Carcinoma.Pathol Oncol Res. 2018 Jul 19. Read more>>
  • Mitchell A et al. Human induced pluripotent stem cells ameliorate hyperoxia-induced lung injury in a mouse model. Am J Transl Res. 2020 Jan 15;12(1):292-307.Read more>>
  • Takanori Watanabe et al. Aquaporin 3 Expression in Endometrioid Carcinoma of the Uterine Body Correlated With Early Stage and Lower Grade. Pathol Oncol Res. 2020 Oct;26(4):2247-2253.Read more>>
  • Liu X. et al. Effects of Different Ligands in the Notch Signaling Pathway on the Proliferation and Transdifferentiation of Primary Type II Alveolar Epithelial Cells.. Front Pediatr. 2020 Aug;8:452-452Read more>>
  • Haifa Alkhalifa. et al. Inhibition of aquaporins as a potential adjunct to breast cancer cryotherapy. Oncol Lett. 2021 Jun;21(6):1-11Read more>>
  • Shota Mitsuboshi. et al. A novel alveolar epithelial cell sheet fabricated under feeder-free conditions for potential use in pulmonary regenerative therapy. Regen Ther. 2022 Mar;19:113Read more>>
  • Luo, Wenping. et al. Reversely immortalized mouse salivary gland cells presented a promising metabolic and fibrotic response upon BMP9/Gdf2 stimulation. CELL MOL BIOL LETT. 2022 Dec;27(1):1-21Read more>>
VALIDATION IMAGES

Formalin-fixed and paraffin embedded rat kidney labeled with Anti-AQP5 Polyclonal Antibody, Unconjugated (bs-1554R) followed by conjugation to the secondary antibody and DAB staining


A549 cells probed with AQP5 Polyclonal Antibody, Unconjugated (bs-1554R) at 1:20 for 30 minutes followed by incubation with a conjugated secondary (bs-0295G-PE) (green) for 30 minutes compared to control cells (blue), secondary only (light blue) and isotype control (orange)


Formalin-fixed and paraffin embedded human kidney labeled with Rabbit Anti-AQP5 Polyclonal Antibody, Unconjugated (bs-1554R) at 1:200 followed by conjugation to the secondary antibody and DAB staining\n


Formalin-fixed and paraffin embedded rat lung labeled with Rabbit Anti-AQP5 Polyclonal Antibody, Unconjugated (bs-1554R) at 1:200 followed by conjugation to the secondary antibody and DAB staining\n