bs-1571R
[Primary Antibody]
F-Actin Polyclonal Antibody
DATASHEET
Host: Rabbit
Target Protein: F-Actin
Immunogen Range: 1-50/374
Clonality: Polyclonal
Isotype: IgG
Entrez Gene: 60
Swiss Prot: P52907
Source: KLH conjugated synthetic peptide derived from human F-actin capping protein alpha subunit
Purification: Purified by Protein A.
Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Background:
Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. F-actin-capping proteins bind in a Ca2+-independent manner to the fast growing ends of actin filaments (barbed end) thereby blocking the exchange of subunits at these ends. Unlike other capping proteins (such as gelsolin and severin), these proteins do not sever actin filaments. May play a role in the formation of epithelial cell junctions.
Size: 100ul
Concentration: 1ug/ul
Applications:
WB(1:300-5000)
ELISA(1:500-1000)
FCM(1:20-100)
IHC-P(1:200-400)
IF(IHC-P)(1:50-200)
IF(ICC)(1:50-200)
Predicted Molecular Weight: 33
Cross Reactive Species:
Human
Mouse
Rat
Dog
Chicken
Predicted Cross Reactive Species:
Cow
Sheep
Pig
For research use only. Not intended for diagnostic or therapeutic use.
PRODUCT SPECIFIC PUBLICATIONS
- Zhong, Weiliang, et al. "Regulation of Fibrochondrogenesis of Mesenchymal Stem Cells in an Integrated Microfluidic Platform Embedded with Biomimetic Nanofibrous Scaffolds." PLOS ONE 8.4 (2013): e61283.Read more>>
- Zhong, Weiliang, et al. "An integrated microfluidic device for characterizing chondrocyte metabolism in response to distinct levels of fluid flow stimulus."Microfluidics and Nanofluidics: 1-11.Read more>>
- Kang, Hongyan, et al. "A Potential Gravity-Sensing Role of Vascular Smooth Muscle Cell Glycocalyx in Altered Gravitational Stimulation." Astrobiology (2013).Read more>>
- Zhao, Ran, et al. "Hepatoma cell line HepG2. 2.15 demonstrates distinct biological features compared with parental HepG2." World journal of gastroenterology: WJG 17.9 (2011): 1152.Read more>>
- Xie, Chaoming, Xiong Lu, and Kefeng Wang. "Pulse Electrochemical Synthesis of Spherical Hydroxyapatite and Silver Nanoparticles Mediated by the Polymerization of Polypyrrole on Metallic Implants for Biomedical Applications." Particle & Particle Systems Characterization (2015).Read more>>
- Yuan, Lin, et al. "Deubiquitylase OTUD3 regulates PTEN stability and suppresses tumorigenesis." Nature Cell Biology (2015).Read more>>
- Deguchi, Takahiro. "Development of 3D super-resolution tomographic STED microscopy and its application to studies on bone resorption." (2015).Read more>>
- Chen, S. M., et al. "Cofilin is correlated with sperm quality and influences sperm fertilizing capacity in humans." Andrology (2016).Read more>>
- Siar, Chong Huat, et al. "Invadopodia proteins, cortactin, N‐WASP and WIP differentially promote local invasiveness in ameloblastoma." Journal of Oral Pathology & Medicine (2016).Read more>>
- Du, Ailin, et al. "Damage of hippocampal neurons in rats with chronic alcoholism." Neural regeneration research 9.17 (2014): 1610.Read more>>
- Zhang, Pengfei, et al. "Proteome analysis of egg yolk after exposure to zinc oxide nanoparticles." Theriogenology (2017).Read more>>
- Li, Haihong, et al. "Human eccrine sweat gland cells reconstitute polarized spheroids when subcutaneously implanted with Matrigel in nude mice." Journal of molecular histology 47.5 (2016): 485-490.Read more>>
- Guo, Xiliang, and Xuezheng Liu. "Nogo receptor knockdown and ciliary neurotrophic factor attenuate diabetic retinopathy in streptozotocin-induced diabetic rats." Molecular Medicine Reports. (2017).Read more>>
- Clark, Nicolette M., et al. "The human, F-actin-based cytoskeleton as a mutagen sensor." Cancer Cell International 17.1 (2017): 121.Read more>>
- Clark et al. The human, F-actin-based cytoskeleton as a mutagen sensor. (2017) Cancer.Cell.Int. 17:121Read more>>
- Murakami et al. Roles of Ras Homolog A in Invasive Ductal Breast Carcinoma. (2016) Acta.Histochem.Cytochem. 49:131-140Read more>>
- Du et al. Actin filament reorganization is a key step in lung inflammation induced by systemic inflammatory response syndrome. (2012) Am.J.Respir.Cell.Mol.Biol. 47:597-603Read more>>
- Yang et al. Rab5-mediated VE-cadherin internalization regulates the barrier function of the lung microvascular endothelium. (2015) Cell.Mol.Life.Sci. 72:4849-66Read more>>
- Jing et al. Expression of immunoglobulin G in human podocytes, and its role in cell viability and adhesion. (2018) Int.J.Mol.Med. 41:3296-3306Read more>>
- Bwalya et al. Pentosan polysulfate inhibits IL-1β-induced iNOS, c-Jun and HIF-1α upregulation in canine articular chondrocytes. (2017) PLoS.One. 12:e0177144Read more>>
- Chen et al. Cofilin is correlated with sperm quality and influences sperm fertilizing capacity in humans. (2016) Andrology. 4:1064-1072Read more>>
- Okada T et al. Nanoscale imaging of the adhesion core including integrin β1 on intact living cells using scanning electron-assisted dielectric-impedance microscopy. (2018) PLOS ONE, 13(9), e0204133. Read more>>
- Okada and Ogura Nanoscale imaging of the adhesion core including integrin β1 on intact living cells using scanning electron-assisted dielectric-impedance microscopy. (2018) PLoS.One. 13:e0204133Read more>>
- Li X et al. LncRNA FTX inhibits hippocampal neuron apoptosis by regulating miR-21-5p/SOX7 axis in a rat model of temporal lobe epilepsy.Biochem Biophys Res Commun. 2019 Apr 23;512(1):79-86.Read more>>
- Yagi H et al. Gα13-mediated LATS1 down-regulation contributes to epithelial-mesenchymal transition in ovarian cancer. FASEB J. 2019 Sep 28:fj201901278R.Read more>>
- Shiozawa M et al. Biological reaction control using topography regulation of nanostructured titanium. Sci Rep. 2020 Feb 12;10(1):2438. Read more>>
- Shiozawa M et al. Biological reaction control using topography regulation of nanostructured titanium. Sci Rep. 2020 Feb 12;10(1):2438. Read more>>
- Tomoko Okada. et al. Scanning Electron-Assisted Dielectric Microscopy Reveals Autophagosome Formation by LC3 and ATG12 in Cultured Mammalian Cells. Int J Mol Sci. 2021 Jan;22(4):1834Read more>>
- areera Qamar. et al. Intracellular Delivery of Active Proteins by Polyphosphazene Polymers. Pharmaceutics. 2021 Feb;13(2):249Read more>>
- Alisha Wehdnesday Bernard Reyes. et al. Formyl peptide receptor 2 (FPR2) antagonism is a potential target for the prevention of Brucella abortus 544 infection. Immunobiology. 2021 May;226:152073Read more>>
- Taketo Suzuki. et al. Vascular Shutdown by Photodynamic Therapy Using Talaporfin Sodium. Cancers. 2020 Sep;12(9):2369Read more>>
- Tran X. N. Huy. et al. Cobalt (II) Chloride Regulates the Invasion and Survival of Brucella abortus 544 in RAW 264.7 Cells and B6 Mice. PATHOGENS. 2022 May;11(5):596Read more>>
- Jiangliu Yang. et al. Baicalin Attenuates PantonCValentine Leukocidin (PVL)-Induced Cytoskeleton Rearrangement via Regulating the RhoA/ROCK/LIMK and PI3K/AKT/GSK-3 Pathways in Bovine Mammary Epithelial Cells. INT J MOL SCI. 2023 Jan;24(19):1452Read more>>
- Jiangtao Zhou. et al. An Inulin-Type Fructan CP-A from Codonopsis pilosula Alleviated 5-Fluorouracil-Induced Intestinal Mucositis via the ERK/MLCK/MLC2 Pathway and Regulation of Gut Microbiota. PHARMACEUTICALS-BASE. 2024 Mar;17(3):297Read more>>
VALIDATION IMAGES
Formalin-fixed and paraffin embedded rat skeletal muscle labeled with Anti-F-Actin Polyclonal Antibody, Unconjugated (bs-1571R) at 1:200, followed by conjugation to the secondary antibody and DAB staining
L1 and L2 rat skeletal muscle lysates probed: : Anti-F-Actin (bs-1571R) at 1:200 in 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 90min in RT. Predicted and observed band size: 42kDa
A549 cell lysates probed with Anti-F-Actin Polyclonal Antibody, Unconjugated (bs-1571R) at 1:300 overnight at 4˚C. Followed by a conjugated secondary antibody (bs-0295G-HRP) at 1:5000 for 90 min at 37˚C.