Host: Rabbit
Target Protein: PUMA
Immunogen Range: 131-180/193
Clonality: Polyclonal
Isotype: IgG
Entrez Gene: 27113
Swiss Prot: Q96PG8
Source: KLH conjugated synthetic peptide derived from human PUMA
Purification: Purified by Protein A.
Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Background:
Size: 100ul
Concentration: 1ug/ul
Applications:
WB(1:300-5000)
ELISA(1:500-1000)
FCM(1:20-100)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)
IP(1-2ug)
Predicted Molecular Weight: 21
Cross Reactive Species:
Human
Rat
Pig
Predicted Cross Reactive Species:
Mouse
Dog
Cow
For research use only. Not intended for diagnostic or therapeutic use.
- Zhang, Pei, et al. "Epithelial-mesenchymal transition is necessary for acquired resistance to cisplatin and increases the metastatic potential of nasopharyngeal carcinoma cells." International Journal of Molecular Medicine (2013).Read more>>
- Qiu, Shi, et al. "A small peptide derived from p53 linker region can resume the apoptotic activity of p53 by sequestering iASPP with p53." Cancer Letters 356.2 (2015): 910-917.Read more>>
- Su et al. Anticancer bioactive peptides suppress human colorectal tumor cell growth and induce apoptosis via modulating the PARP-p53-Mcl-1 signaling pathway. (2015) Acta.Pharmacol.Si. 36:1514-9Read more>>
- Liu S et al. PKCδ contributes to oxidative stress-induced apoptosis in porcine ovarian granulosa cells via activating JNK. Theriogenology. 2019 Jun;131:89-95.Read more>>
- Su C et al. Effect of electroacupuncture at the ST36 and GB39 acupoints on apoptosis by regulating the p53 signaling pathway in adjuvant arthritis rats. Mol Med Rep. 2019 Sep 11. Read more>>
- Ding Wang. et al. GRIM-19 inhibits proliferation and induces apoptosis in a p53-dependent manner in colorectal cancer cells through the SIRT7/PCAF/MDM2 axis. Exp Cell Res. 2021 Oct;407:112799Read more>>
- Chen Yingtong. et al. p53 directly downregulates the expression of CDC20 to exert anti-tumor activity in mantle cell lymphoma. EXP HEMATOL ONCOL. 2023 Dec;12(1):1-23Read more>>
Formalin-fixed and paraffin embedded rat brain labeled with Rabbit Anti BBC3/PUMA Polyclonal Antibody, Unconjugated (bs-1573R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
U87MG cells probed with Puma Polyclonal Antibody, Unconjugated (bs-1573R) at 1:50 for 30 minutes followed by incubation with a conjugated secondary (PE Conjugated) (green) for 30 minutes compared to control cells (blue), secondary only (light blue) and isotype control (orange).
K562 lysates probed with PUMA Polyclonal Antibody, unconjugated (bs-1573R) at 1:300 overnight at 4°C followed by a conjugated secondary antibody at 1:10000 for 60 minutes at 37°C.