bs-1573R [Primary Antibody]
PUMA POLYCLONAL ANTIBODY
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: PUMA

Immunogen Range: 131-180/193

Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 27113

Swiss Prot: Q96PG8

Source: KLH conjugated synthetic peptide derived from human PUMA

Purification: Purified by Protein A.

Storage Buffer: Aqueous buffered solution containing 1% BSA, 50% glycerol and 0.09% sodium azide.

Storage: Store at -20°C for 12 months..

Background:

Essential mediator of p53/TP53-dependent and p53/TP53-independent apoptosis. Isoform 3 fails to show any growth-inhibitory or apoptotic activity.

Size: 100ul

Concentration: 1ug/ul

Predicted Molecular Weight: 21

Cross Reactive Species: Human
Rat
Pig

Predicted Cross Reactive Species: Mouse
Dog
Cow

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Zhang, Pei, et al. "Epithelial-mesenchymal transition is necessary for acquired resistance to cisplatin and increases the metastatic potential of nasopharyngeal carcinoma cells." International Journal of Molecular Medicine (2013).Read more>>
  • Qiu, Shi, et al. "A small peptide derived from p53 linker region can resume the apoptotic activity of p53 by sequestering iASPP with p53." Cancer Letters 356.2 (2015): 910-917.Read more>>
  • Su et al. Anticancer bioactive peptides suppress human colorectal tumor cell growth and induce apoptosis via modulating the PARP-p53-Mcl-1 signaling pathway. (2015) Acta.Pharmacol.Si. 36:1514-9Read more>>
  • Liu S et al. PKCδ contributes to oxidative stress-induced apoptosis in porcine ovarian granulosa cells via activating JNK. Theriogenology. 2019 Jun;131:89-95.Read more>>
  • Su C et al. Effect of electroacupuncture at the ST36 and GB39 acupoints on apoptosis by regulating the p53 signaling pathway in adjuvant arthritis rats. Mol Med Rep. 2019 Sep 11. Read more>>
  • Ding Wang. et al. GRIM-19 inhibits proliferation and induces apoptosis in a p53-dependent manner in colorectal cancer cells through the SIRT7/PCAF/MDM2 axis. Exp Cell Res. 2021 Oct;407:112799Read more>>
VALIDATION IMAGES

Formalin-fixed and paraffin embedded rat brain labeled with Rabbit Anti BBC3/PUMA Polyclonal Antibody, Unconjugated (bs-1573R) at 1:200 followed by conjugation to the secondary antibody and DAB staining


U87MG cells probed with Puma Polyclonal Antibody, Unconjugated (bs-1573R) at 1:50 for 30 minutes followed by incubation with a conjugated secondary (PE Conjugated) (green) for 30 minutes compared to control cells (blue), secondary only (light blue) and isotype control (orange).


K562 lysates probed with PUMA Polyclonal Antibody, unconjugated (bs-1573R) at 1:300 overnight at 4°C followed by a conjugated secondary antibody at 1:10000 for 60 minutes at 37°C.