bs-1586R [Primary Antibody]
VEGF-C Polyclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: VEGF-C

Immunogen Range: 321-415/415


Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 22341

Swiss Prot: P97953

Source: KLH conjugated synthetic peptide derived from mouse VEGF-C

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Background:

Growth factor active in angiogenesis, and endothelial cell growth, stimulating their proliferation and migration and also has effects on the permeability of blood vessels. May function in angiogenesis of the venous and lymphatic vascular systems during embryogenesis, and also in the maintenance of differentiated lymphatic endothelium in adults. Binds and activates VEGFR-2 (KDR/FLK1) and VEGFR-3 (FLT4) receptors.

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)
ELISA(1:500-1000)
FCM(1:20-100)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)

Predicted Molecular Weight: 46


Cross Reactive Species: Human
Mouse
Rat

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Zhuo, Wei, et al. "The CXCL12?CCXCR4 Chemokine Pathway: A Novel Axis Regulates Lymphangiogenesis."Clinical Cancer Research 18.19 (2012): 5387-5398.lRead more>>
  • He, Ting, et al. "Tumor cell-secreted angiogenin induces angiogenic activity of endothelial cells by suppressing miR-542-3p." Cancer Letters (2015).Read more>>
  • Alunno, Alessia, et al. "Mobilization of lymphatic endothelial precursor cells and lymphatic neovascularization in primary Sjögrens syndrome." Journal of Cellular and Molecular Medicine (2016).Read more>>
  • Cialdai, Francesca, et al. "Modeled Microgravity Affects Fibroblast Functions Related to Wound Healing." Microgravity Science and Technology: 1-12.Read more>>
  • Shin et al. Expression of vascular endothelial growth factor-C (VEGF-C) and its receptor (VEGFR-3) in the glial reaction elicited by human mesenchymal stem cell engraftment in the normal rat brain. (2015) J.Histochem.Cytochem. 63:170-80Read more>>
  • Alunno et al. Mobilization of lymphatic endothelial precursor cells and lymphatic neovascularization in primary Sjögren's syndrome. (2016) J.Cell.Mol.Med. 20:613-22Read more>>
  • Ferrão JSP et al. Vascular endothelial growth factor C treatment for mouse hind limb lymphatic revascularization. Vet Med Sci. 2019 Feb 11. Read more>>
  • Li Li. et al. Circ_LPAR3 promotes the progression of oral squamous cell carcinoma (OSCC). Biochem Bioph Res Co. 2021 DecRead more>>
  • Michiko Jo. et al. Impact of Goreisan components on rat mesenteric collecting lymphatic vessel pumping. MICROCIRCULATION. 2022 Oct;:e12788Read more>>
VALIDATION IMAGES

Paraformaldehyde-fixed, paraffin embedded Rat small intestine; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with VEGF-C Polyclonal Antibody, Unconjugated (bs-1586R) at 1:400 overnight at 4°C, DAB staining.


Lane 1: Mouse Lymph node lysates; Lane 2: Mouse Kidney lysates; Lane 3: Rat Thymus lysates; Lane 4: Rat Lymph node lysates; Lane 5: Rat Kidney lysates probed with VEGF-C Polyclonal Antibody, Unconjugated (bs-1586R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.


HepG2 cells were fixed with 4% PFA for 10min at room temperature,permeabilized with 0.1% PBST for 20 min at room temperature, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with VEGF-C Polyclonal Antibody(bs-1586R)at 1:100 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).