bs-1610R
[Primary Antibody]
CK7 Polyclonal Antibody
DATASHEET
Host: Rabbit
Target Protein: CK7
Immunogen Range: 251-350/469
Clonality: Polyclonal
Isotype: IgG
Entrez Gene: 3855
Swiss Prot: P08729
Source: KLH conjugated synthetic peptide derived from human of human CK7
Purification: Purified by Protein A.
Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Background:
Blocks interferon-dependent interphase and stimulates DNA synthesis in cells. Involved in the translational regulation of the human papillomavirus type 16 E7 mRNA (HPV16 E7).
Size: 100ul
Concentration: 1ug/ul
Applications:
WB(1:300-5000)
ELISA(1:500-1000)
FCM(1:20-100)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)
Predicted Molecular Weight: 54
Cross Reactive Species:
Human
Mouse
Predicted Cross Reactive Species: Rat
For research use only. Not intended for diagnostic or therapeutic use.
PRODUCT SPECIFIC PUBLICATIONS
- Hu, Bin, et al. "IFN‐γ Inhibits Osteopontin Expression in Human Decidual Stromal Cells and can be Attenuated by 1α, 25‐Dihydroxyvitamin D3." American Journal of Reproductive Immunology 68.4 (2012): 353-361.Read more>>
- Uchikura, Yuka, et al. "Extranuclear Translocation of High-Mobility Group A1 Reduces the Invasion of Extravillous Trophoblasts Involved in the Pathogenesis of Preeclampsia: New Aspect of High-Mobility Group A1." Reproductive Sciences (2017): 1933719117697254.Read more>>
- Ding, Dah-Ching, et al. "Expression of CD133 in endometrial cancer cells and its implications." Journal of Cancer 8.11 (2017): 2142-2153.Read more>>
- Matsubara et al. Immune activation during the implantation phase causes preeclampsia-like symptoms via the CD40-CD40 ligand pathway in pregnant mice. (2016) Hypertens.Re. 39:407-14Read more>>
- Hu et al. Phosphoinositide 3-Kinase (PI3K) Subunit p110δ Is Essential for Trophoblast Cell Differentiation and Placental Development in Mouse. (2016) Sci.Re. 6:28201Read more>>
- Ding et al. Expression of CD133 in endometrial cancer cells and its implications. (2017) J.Cancer. 8:2142-2153Read more>>
- Chen et al. Silencing of Paternally Expressed Gene 10 Inhibits Trophoblast Proliferation and Invasion. (2015) PLoS.One. 10:e0144845Read more>>
- Mariam A. Fouad. et al. Epigenetic immunomodulatory effect of eugenol and astaxanthin on doxorubicin cytotoxicity in hormonal positive breast Cancer cells. Bmc Pharmacol Toxico. 2021 Dec;22(1):1-15Read more>>
- Yu-Hsun Chang. et al. A clear cancer cell line (150057) derived from human endometrial carcinoma harbors two novel mutations. Bmc Cancer. 2020 Dec;20(1):1-12Read more>>
- Yunsheng Wang. et al. A rare case of giant panda cancer: Pancreatic ductal adenocarcinoma. Animal Models and Experimental Medicine. 2022 NovRead more>>
VALIDATION IMAGES
Formalin-fixed and paraffin embedded human laryngocarcinoma labeled with Anti-CK7 Polyclonal Antibody, Unconjugated (bs-1610R) at 1:300 followed by conjugation to the secondary antibody and DAB staining.
Formalin-fixed and paraffin embedded human laryngocarcinoma labeled with Anti-CK7 Polyclonal Antibody, Unconjugated (bs-1610R) at 1:300 followed by conjugation to the secondary antibody and DAB staining.
Formalin-fixed and paraffin embedded human lung carcinoma labeled with Anti-CK7 Polyclonal Antibody, Unconjugated (bs-1610R) at 1:300 followed by conjugation to the secondary antibody and DAB staining.
Formalin-fixed and paraffin embedded human lung carcinoma labeled with Anti-CK7 Polyclonal Antibody, Unconjugated (bs-1610R) at 1:300 followed by conjugation to the secondary antibody and DAB staining.
Formalin-fixed and paraffin embedded human esophageal carcinoma labeled with Rabbit Anti-CK7 Polyclonal Antibody, Unconjugated (bs-1610R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin embedded human lung cancer labeled with Anti-CK7 (Human) Polyclonal Antibody, Unconjugated (bs-1610R) followed by conjugation to the secondary antibody and DAB staining
Hela cells were fixed with 4% PFA for 10min at room temperature,permeabilized with 90% ice-cold methanol for 20 min at -20℃, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with CK7 Polyclonal Antibody(bs-1610R)at 1:100 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).
Paraformaldehyde-fixed, paraffin embedded (Rat bladder); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (CK7) Polyclonal Antibody, Unconjugated (bs-1610R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (Rat bladder); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (CK7) Polyclonal Antibody, Unconjugated (bs-1610R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Lane 1: Mouse Urinary bladder tissue lysates; Lane 2: Mouse Breast tissue lysates; Lane 3: Mouse Placenta tissue lysates; Lane 4: Mouse trachea tissue lysates; Lane 5: Rat Urinary bladder tissue lysates; Lane 6: Rat Placenta tissue lysates; Lane 7: Human Hela cell lysates; Lane 8: Human HepG2 cell lysates; Lane 9: Human Siha cell lysates; Lane 10: Human Huvec cell lysates probed with CK7 Polyclonal Antibody, Unconjugated (bs-1610R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.