bs-16151R-FITC [Conjugated Primary Antibody]
FMNL3 Polyclonal Antibody, FITC Conjugated
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: FMNL3

Specificity: Possible cross-reactivity with FMNL2 and FMNL1

Immunogen Range: 951-1028/1028


Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 91010

Swiss Prot: Q8IVF7

Source: KLH conjugated synthetic peptide derived from human FMNL3

Purification: Purified by Protein A.

Storage Buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Background:

The protein encoded by this gene contains a formin homology 2 domain and has high sequence identity to the mouse Wbp3 protein. Two alternative transcripts encoding different isoforms have been described. [provided by RefSeq, Jul 2008]

Conjugation: FITC

Excitation/ Emission: 494nm/518nm

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)
FCM(1:20-100)

Predicted Molecular Weight: 117 kDa


Cross Reactive Species: Human
Mouse
Rat

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Pan ZN et al. RAB7 GTPase regulates actin dynamics for DRP1\mediated mitochondria function and spindle migration in Mouseoocyte meiosis. FASEB J. 2020 Jul;34(7):9615-9627.Read more>>
  • Pan Z et al. RAB7 GTPase regulates actin dynamics for DRP1\mediated mitochondria function and spindle migration in Mouseoocyte meiosis. FASEB J. 2020 Jul;34(7):9615-9627.Read more>>
VALIDATION IMAGES

Molt-4 cells were fixed with 4% PFA for 10min at room temperature, permeabilized with 20% PBST for 20 min at room temperature, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with FMNL3 Polyclonal Antibody, FITC Conjugated (bs-16151R-FITC) at 1:100 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2% BSA in PBS. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green) and isotype control (orange).