Host: Rabbit
Target Protein: Smad1 + Smad5
Immunogen Range: 361-465/465
Clonality: Polyclonal
Isotype: IgG
Entrez Gene: 4090, 4086
Source: KLH conjugated synthetic peptide derived from human Smad 5
Purification: Purified by Protein A.
Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Background:
Size: 100ul
Concentration: 1ug/ul
Applications:
ELISA(1:500-1000)
FCM(1:20-100)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)
ICC(1:100-500)
Predicted Molecular Weight: 52
Cross Reactive Species: Human
Predicted Cross Reactive Species:
Mouse
Rat
Dog
Cow
Pig
Horse
Rabbit
For research use only. Not intended for diagnostic or therapeutic use.
- Qu et al. MiR-199a-3p suppresses proliferation and invasion of prostate cancer cells by targeting Smad1. (2017) Oncotarge. 8:52465-52473Read more>>
- Wei et al. Smurf1 inhibits integrin activation by controlling Kindlin-2 ubiquitination and degradation. (2017) J.Cell.Bio. 216:1455-1471Read more>>
Formalin-fixed and paraffin embedded human lung carcinoma labeled with Anti-Smad1 Polyclonal Antibody, Unconjugated (bs-1619R) at 1:200 followed by conjugation to the secondary antibody and DAB staining\n
Hela cells were fixed with 4% PFA for 10min at room temperature,permeabilized with 90% ice-cold methanol for 20 min at -20℃,and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with Smad1 + Smad5 Antibody(bs-1619R)at 1:100 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).