DATASHEET
Host:
Rabbit
Target Protein:
GLIS3
Immunogen Range:
101-200/775
Clonality:
Polyclonal
Isotype:
IgG
Entrez Gene:
169792
Swiss Prot:
Q8NEA6
Source:
KLH conjugated synthetic peptide derived from human GLIS3
Purification:
Purified by Protein A.
Storage Buffer:
0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage:
Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Background:
Acts as both a repressor and activator of transcription. Binds to the consensus sequence 5'-GACCACCCAC-3' (By similarity).
PRODUCT SPECIFIC PUBLICATIONS
- Zhuohang Liu. et al. GLIS Family Zinc Finger 3 Promoting Cell Malignant Behaviors and NF-B Signaling in Glioma. Brain Res. 2021 Aug;:147623Read more>>
- Hong-Bo YANG. et al. The compensatory increase of GLIS3 inhibited neuronal apoptosis through regulating MOB1b: a possible strategy for the functional recovery after spinal cord injury. EXP ANIM TOKYO. 2023 Aug 12Read more>>
VALIDATION IMAGES
Formalin-fixed and paraffin embedded rat kidney labeled with Rabbit Anti-GLIS3 Polyclonal Antibody, Unconjugated (bs-16249R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin embedded human kidney labeled with Rabbit Anti-GLIS3 Polyclonal Antibody, Unconjugated (bs-16249R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Lane 1: Human U251 cell lysates; Lane 2: Human U87MG cell lysates; Lane 3: Human A549 cell lysates; Lane 4: Human 293T cell lysates; Lane 5: Human MDA-MB-231 cell lysates probed with GLIS3 Polyclonal Antibody, Unconjugated (bs-16249R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.