bs-16311R [Primary Antibody]
GRF2 Polyclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: GRF2

Immunogen Range: 20-120/1237


Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 5924

Swiss Prot: O14827

Source: KLH conjugated synthetic peptide derived from human GRF2

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Background:

Functions as a calcium-regulated nucleotide exchange factor activating both Ras and RAC1 through the exchange of bound GDP for GTP. Preferentially activates HRAS in vivo compared to RRAS based on their different types of prenylation. Functions in synaptic plasticity by contributing to the induction of long term potentiation.

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)
ELISA(1:500-1000)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)
ICC(1:100-500)

Predicted Molecular Weight: 140


Cross Reactive Species: Mouse

Predicted Cross Reactive Species: Human
Rat
Cow
Sheep

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Mouse placenta lysate probed with Rabbit Anti-GRF2 Polyclonal Antibody, Unconjugated (bs-16311R) at 1:300 in 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:5000 for 90min at RT.


Lane 1: Human MCF-7 cell lysates; Lane 2: Human HL-60 cell lysates; Lane 3: Human JAR cell lysates; Lane 4: Human HeLa cell lysates probed with GRF2 Polyclonal Antibody, Unconjugated (bs-16311R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.