bs-16376R [Primary Antibody]
SMAD1 Polyclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: SMAD1

Specificity: This antibody may cross react with AKAP6 due to a few animo acid sequence similarity.

Immunogen Range: 151-250/465


Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 4086

Swiss Prot: Q15797

Source: KLH conjugated synthetic peptide derived from human SMAD1

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Background:

Transcriptional modulator activated by BMP (bone morphogenetic proteins) type 1 receptor kinase. SMAD1 is a receptor-regulated SMAD (R-SMAD). SMAD1/OAZ1/PSMB4 complex mediates the degradation of the CREBBP/EP300 repressor SNIP1. May act synergistically with SMAD4 and YY1 in bone morphogenetic protein (BMP)-mediated cardiac-specific gene expression.

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)
ELISA(1:500-1000)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)
ICC(1:100-500)

Predicted Molecular Weight: 51


Cross Reactive Species: Human
Mouse
Rat

Predicted Cross Reactive Species: Chicken

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Yang et al. miR-1307-3p suppresses the chondrogenic differentiation of human adipose-derived stem cells by targeting BMPR2. (2018) Int.J.Mol.Med. 42:3115-3124Read more>>
VALIDATION IMAGES

Formalin-fixed and paraffin embedded human breast cancer labeled with SMAD1 Polyclonal Antibody, Unconjugated (bs-16376R) at 1:200 followed by conjugation to the secondary antibody and DAB staining


Line 1, mouse heart lysates;line 2, mouse muscle lysates; probed with SMAD1 Polyclonal Antibody, Unconjugated (bs-16376R) at 1:300 in 4˚C overnight. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:5000 for 90min at 37˚C.