bs-1638R [Primary Antibody]
CD59 Polyclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: CD59

Immunogen Range: 52-100/128


Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 966

Swiss Prot: P13987

Source: KLH conjugated synthetic peptide derived from human CD59

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Background:

Potent inhibitor of the complement membrane attack complex (MAC) action. Acts by binding to the C8 and/or C9 complements of the assembling MAC, thereby preventing incorporation of the multiple copies of C9 required for complete formation of the osmolytic pore. This inhibitor appears to be species-specific. Involved in signal transduction for T-cell activation complexed to a protein tyrosine kinase. The soluble form from urine retains its specific complement binding activity, but exhibits greatly reduced ability to inhibit MAC assembly on cell membranes.

Size: 100ul

Concentration: 1ug/ul

Applications: ELISA(1:500-1000)
FCM(1:20-100)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)

Predicted Molecular Weight: 9


Cross Reactive Species: Human

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Lingzi Feng. et al. A Closed-Loop Autologous Erythrocyte-Mediated Delivery Platform for Diabetic Nephropathy Therapy. NANOMATERIALS-BASEL. 2022 Jan;12(20):3556Read more>>
VALIDATION IMAGES

Formalin-fixed and paraffin embedded human placenta labeled with Anti-CD59 Polyclonal Antibody, Unconjugated (bs-1638R) followed by conjugation to the secondary antibody and DAB staining


293T cells probed with CD59 Polyclonal Antibody, unconjugated (bs-1638R) at 1:100 dilution for 30 minutes compared to control cells (blue) and isotype control (orange)