Host: Rabbit
Target Protein: XBP1
Specificity: XBP-1 antibody will recognize both isoforms, may also interact with chicken
Immunogen Range: 51-150/261
Clonality: Polyclonal
Isotype: IgG
Entrez Gene: 7494
Swiss Prot: P17861
Source: KLH conjugated synthetic peptide derived from human XBP-1
Purification: Purified by Protein A.
Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Background:
Size: 100ul
Concentration: 1ug/ul
Applications:
WB(1:300-5000)
ELISA(1:500-1000)
FCM(1:20-100)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)
Predicted Molecular Weight: 29/40
Cross Reactive Species:
Human
Mouse
Predicted Cross Reactive Species:
Rat
Cow
Chicken
For research use only. Not intended for diagnostic or therapeutic use.
- Li, Xiaoyan, Fang Han, and Yuxiu Shi. "IRE1α-XBP1 Pathway Is Activated Upon Induction of Single-Prolonged Stress in Rat Neurons of the Medial Prefrontal Cortex." Journal of Molecular Neuroscience (2015): 1-10.Read more>>
- Li,et al.Silver nanoparticles induce SH-SY5Y cell apoptosis via endoplasmic reticulum- and mitochondrial pathways that lengthen endoplasmic reticulum-mitochondria contact sites and alter inositol-3-phosphate receptor function.(2018) Toxicology Letters. 285:156-167.Read more>>
- Qiu,et al.Recombinant human maspin inhibits high glucose-induced oxidative stress and angiogenesis of human retinal microvascular endothelial cells via PI3K/AKT pathway.(2018) Molecular and Cellular Biochemistry. :.Read more>>
- Wang L et al. Radioprotective effect of Hohenbuehelia serotina polysaccharides through mediation of ER apoptosis pathway in vivo. Int J Biol Macromol. 2019 Apr 15;127:18-26. Read more>>
- Yam Prasad Aryal. et al. Stage-specific expression patterns of ER stress-related molecules in mice molars: Implications for tooth development. Gene Expr Patterns. 2020 Sep;37:11913Read more>>
- Chunyue Wang. et al. Neuroprotective effects of verbascoside against Alzheimers disease via the relief of endoplasmic reticulum stress in A-exposed U251 cells and APP/PS1 mice. J Neuroinflamm. 2020 Dec;17(1):1-16Read more>>
- Lei Zhou. et al. Silencing circ?BIRC6 inhibits the proliferation, invasion, migration and epithelial?mesenchymal transition of bladder cancer cells by targeting the miR?495?3p/XBP1 signaling axis. Mol Med Rep. 2021 Nov;24(5):1-11Read more>>
- Xueying Zhang. et al. Scutellarin ameliorates hepatic lipid accumulation by enhancing autophagy and suppressing IRE1/XBP1 pathway. 2021 Dec 02Read more>>
- Elisa Martino. et al. Milk Exosomal miR-27b Worsen Endoplasmic Reticulum Stress Mediated Colorectal Cancer Cell Death. NUTRIENTS. 2022 Jan;14(23):5081Read more>>
L1 mouse embryo lysate L2 mouse liver lysates probed with Anti XBP1 Polyclonal Antibody, Unconjugated (bs-1668R) at 1:200 overnight at 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 for 90 min at RT. Predicted band 29kD. Observed band size: 29kD
Formalin-fixed and paraffin embedded human cervical tissue labeled with Anti-XBP-1 Polyclonal Antibody, Unconjugated (bs-1668R) followed by conjugation to the secondary antibody and DAB staining
Lane 1: Mouse Large intestine lysates; Lane 2: Mouse Liver lysates; Lane 3: Mouse Testis lysates; Lane 4: HepG2 cell lysates probed with XBP1 Polyclonal Antibody, Unconjugated (bs-1668R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
HepG2 cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37°C for 20 min; Antibody incubation with (XBP1) polyclonal Antibody, Unconjugated (bs-1668R) 1:100, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.
Lane 1: Mouse Skin Lysates; Lane 2: Mouse Testis Lysates; Lane 3: Rat Skin Lysates; Lane 4: Rat Testis Lysates. Probed with XBP1 polyclonal Antibody, unconjugated (bs-1668R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Lane 1: Mouse Skin tissue lysates; Lane 2: Rat Skin tissue lysates; Lane 3: Rat Testis tissue lysates probed with XBP1 Polyclonal Antibody, Unconjugated (bs-1668R) at 1:500 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.