bs-1668R-Cy3 [Conjugated Primary Antibody]
XBP1 Polyclonal Antibody, Cy3 Conjugated
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: XBP1

Specificity: XBP-1 antibody will recognize both isoforms, may also interact with chicken

Immunogen Range: 51-150/261


Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 7494

Swiss Prot: P17861

Source: KLH conjugated synthetic peptide derived from human XBP-1

Purification: Purified by Protein A.

Storage Buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.

Storage: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Background:

Transcription factor essential for hepatocyte growth, the differentiation of plasma cells, the immunoglobulin secretion, and the unfolded protein response (UPR). Acts during endoplasmic reticulum stress (ER) by activating unfolded protein response (UPR) target genes via direct binding to the UPR element (UPRE). Binds DNA preferably to the CRE-like element 5'-GATGACGTG[TG]N(3)[AT]T-3', and also to some TPA response elements (TRE). Binds to the HLA DR-alpha promoter. Binds to the Tax-responsive element (TRE) of HTLV-I.

Conjugation: Cy3

Excitation/ Emission: 512,550nm/570,615nm

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)
FCM(1:20-100)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)

Predicted Molecular Weight: 29/40


Cross Reactive Species: Human
Mouse

Predicted Cross Reactive Species: Rat
Cow
Chicken

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Li, Xiaoyan, Fang Han, and Yuxiu Shi. "IRE1α-XBP1 Pathway Is Activated Upon Induction of Single-Prolonged Stress in Rat Neurons of the Medial Prefrontal Cortex." Journal of Molecular Neuroscience (2015): 1-10.Read more>>
  • Li,et al.Silver nanoparticles induce SH-SY5Y cell apoptosis via endoplasmic reticulum- and mitochondrial pathways that lengthen endoplasmic reticulum-mitochondria contact sites and alter inositol-3-phosphate receptor function.(2018) Toxicology Letters. 285:156-167.Read more>>
  • Qiu,et al.Recombinant human maspin inhibits high glucose-induced oxidative stress and angiogenesis of human retinal microvascular endothelial cells via PI3K/AKT pathway.(2018) Molecular and Cellular Biochemistry. :.Read more>>
  • Wang L et al. Radioprotective effect of Hohenbuehelia serotina polysaccharides through mediation of ER apoptosis pathway in vivo. Int J Biol Macromol. 2019 Apr 15;127:18-26. Read more>>
  • Yam Prasad Aryal. et al. Stage-specific expression patterns of ER stress-related molecules in mice molars: Implications for tooth development. Gene Expr Patterns. 2020 Sep;37:11913Read more>>
  • Chunyue Wang. et al. Neuroprotective effects of verbascoside against Alzheimers disease via the relief of endoplasmic reticulum stress in A-exposed U251 cells and APP/PS1 mice. J Neuroinflamm. 2020 Dec;17(1):1-16Read more>>
  • Lei Zhou. et al. Silencing circ?BIRC6 inhibits the proliferation, invasion, migration and epithelial?mesenchymal transition of bladder cancer cells by targeting the miR?495?3p/XBP1 signaling axis. Mol Med Rep. 2021 Nov;24(5):1-11Read more>>
  • Xueying Zhang. et al. Scutellarin ameliorates hepatic lipid accumulation by enhancing autophagy and suppressing IRE1/XBP1 pathway. 2021 Dec 02Read more>>
  • Elisa Martino. et al. Milk Exosomal miR-27b Worsen Endoplasmic Reticulum Stress Mediated Colorectal Cancer Cell Death. NUTRIENTS. 2022 Jan;14(23):5081Read more>>
VALIDATION IMAGES