bs-16797R [Primary Antibody]
DOT1L Polyclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: DOT1L

Immunogen Range: 51-150/1739


Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 84444

Swiss Prot: Q8TEK3

Source: KLH conjugated synthetic peptide derived from human DOT1L

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Background:

Histone methyltransferase. Methylates 'Lys-79' of histone H3. Nucleosomes are preferred as substrate compared to free histones. Binds to DNA.

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)
ELISA(1:500-1000)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)
ICC(1:100-500)

Predicted Molecular Weight: 185


Cross Reactive Species: Human
Mouse
Rat
African Green Monkey

Predicted Cross Reactive Species: Cow
Sheep

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Formalin-fixed and paraffin embedded human kidney labeled with Anti-DOT1L Polyclonal Antibody, Unconjugated (bs-16797R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.