bs-1693R [Primary Antibody]
CANX/Calnexin Polyclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: CANX/Calnexin

Immunogen Range: 501-592/592


Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 821

Swiss Prot: P27824

Source: KLH conjugated synthetic peptide derived from human CANX

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Background:

Calcium-binding protein that interacts with newly synthesized glycoproteins in the endoplasmic reticulum. It may act in assisting protein assembly and/or in the retention within the ER of unassembled protein subunits. It seems to play a major role in the quality control apparatus of the ER by the retention of incorrectly folded proteins. Associated with partial T-cell antigen receptor complexes that escape the ER of immature thymocytes, it may function as a signaling complex regulating thymocyte maturation. Additionally it may play a role in receptor-mediated endocytosis at the synapse.

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)
ELISA(1:500-1000)
FCM(1:20-100)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)

Predicted Molecular Weight: 65


Cross Reactive Species: Mouse
Rat

Predicted Cross Reactive Species: Human
Cow
Horse
Chicken
Rabbit

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Correani, Virginia, et al. "Plasma membrane protein profiling in beta‐amyloid‐treated microglia cell line." Proteomics (2017).Read more>>
  • Ming-Zhi Huang. et al. Exosomes from artesunate-treated bone marrow-derived mesenchymal stem cells transferring SNHG7 to promote osteogenesis via TAF15-RUNX2 pathway. REGEN MED. 2022 Oct 02Read more>>
  • Mignogna Giuseppina. et al. Rab11A Depletion in Microglia-Derived Extracellular Vesicle Proteome upon Beta-Amyloid Treatment. CELL BIOCHEM BIOPHYS. 2023 Mar;:1-11Read more>>
VALIDATION IMAGES

Lane 1: Mouse Testis tissue lysates; Lane 2: Mouse Cerebrum tissue lysates; Lane 3: Rat Testis tissue lysates; Lane 4: Rat Cerebrum tissue lysates; Lane 5: Human K562 cell lysates; Lane 6: Human HeLa cell lysates; Lane 7: Human HepG2 cell lysates; Lane 8: Human U-2 OS cell lysates; Lane 9: Human MCF-7 cell lysates; Lane 10: Human A431 cell lysates probed with Calnexin Polyclonal Antibody, Unconjugated (bs-1693R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at room temperature for 60 min.