bs-1698R-A350 [Conjugated Primary Antibody]
ox-LDL Polyclonal Antibody, ALEXA FLUOR® 350 Conjugated
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: ox-LDL

Clonality: Polyclonal

Isotype: IgG

Source: Full length plasma protein (Human)

Purification: Purified by Protein A.

Storage Buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Background:

Low-density lipoprotein (LDL) is the carrier protein for cholesterol in the blood. LDL binds to its receptor on the capillary walls and thereby mediates the uptake and clearence of cholesterol from the circulation. In atherosclerotic lesions oxidatively modified LDL is found and oxidized LDL is specifically recognized and ingested by macrophages via scavenger receptor A and CD36. Oxidized LDL may be a marker of atherosclerosis but the precise changes in oxidized LDL are not well described. Low-density lipoprotein oxidised with Cu2SO4.

Conjugation: ALEXA FLUOR® 350

Excitation/ Emission: 346nm/442nm

Size: 100ul

Concentration: 1ug/ul

Applications: FCM(1:20-100)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)

Predicted Molecular Weight: 31


Cross Reactive Species: Human
Mouse

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Wen, Song, et al. "OxLDL-targeted iron oxide nanoparticles for in vivo MRI detection of perivascular carotid collar induced atherosclerotic lesions in ApoE-deficient mice." Journal of Lipid Research 53.5 (2012): 829-838.Read more>>
  • Martinez, Jennifer, et al. "Molecular characterization of LC3-associated phagocytosis reveals distinct roles for Rubicon, NOX2 and autophagy proteins." Nature Cell Biology (2015).Read more>>
  • Wang et al. Simvastatin ameliorates renal lipidosis through the suppression of renal CXCL16 expression in mice with adriamycin-induced nephropathy. (2016) Int.J.Clin.Exp.Patho. 8:15696-707Read more>>
  • Xia Zhanget al. NEAT1 knockdown suppresses endothelial cell proliferation and induces apoptosis by regulating miR?638/AKT/mTOR signaling in atherosclerosis. Oncol Rep. 2020 Jul;44(1):115-125.Read more>>
  • Song, Bangrong. et al. Differential Expression of LOXL1-AS1 in Coronary Heart Disease and its Regulatory Mechanism in ox-LDL-Induced Human Coronary Artery Endothelial Cell Pyroptosis. 2021 Oct 11Read more>>
  • Fen Gao. et al. Angiotensin II induces the expression of periostin to promote foam cell formation in oxLDL-treated macrophages. Int J Cardiol. 2022 Jan;347:46Read more>>
  • Wang, Xin-Yuan. et al. Accumulation of LDL/ox-LDL in the necrotic region participates in osteonecrosis of the femoral head: a pathological and in vitro study. Lipids Health Dis. 2021 Dec;20(1):1-13Read more>>
  • Takuya Tsumita. et al. The oxidized-LDL/LOX-1 axis in tumor endothelial cells enhances metastasis by recruiting neutrophils and cancer cells. INT J CANCER. 2022 May 24Read more>>
  • Haikuo Li. et al. Comprehensive single-cell transcriptional profiling defines shared and unique epithelial injury responses during kidney fibrosis. CELL METAB. 2022 OcRead more>>
  • Qing Li. et al. Re-Visiting Antioxidant Therapy in Murine Advanced Atherosclerosis with Brussels Chicory, a Typical Vegetable in Mediterranean Diets. NUTRIENTS. 2023 Jan;15(4):832Read more>>
  • Liu Chan-Jung. et al. Increased renal uptake and urine excretion of oxidized LDL is possibly associated with formation of large calcium oxalate nephrolithiasis: a preliminary study. WORLD J UROL. 2023 Mar;:1-8Read more>>
VALIDATION IMAGES