Host: Rabbit
Target Protein: Galectin 9
Immunogen Range: 51-150/353
Clonality: Polyclonal
Isotype: IgG
Entrez Gene: 3965
Source: KLH conjugated synthetic peptide derived from mouse Galectin
Purification: Purified by Protein A.
Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Background:
Size: 100ul
Concentration: 1ug/ul
Applications:
WB(1:300-5000)
ELISA(1:500-1000)
FCM(1:20-100)
IF(IHC-F)(1:50-200)
Predicted Molecular Weight: 35-39
Cross Reactive Species:
Human
Mouse
Predicted Cross Reactive Species:
Rat
Cow
Pig
For research use only. Not intended for diagnostic or therapeutic use.
- Daley, Donnele, et al. "Dectin 1 activation on macrophages by galectin 9 promotes pancreatic carcinoma and peritumoral immune tolerance." Nature Medicine (2017).Read more>>
- Tung-Wei Hsu. et al. Galectin-1-mediated MET/AXL signaling enhances sorafenib resistance in hepatocellular carcinoma by escaping ferroptosis. AGING-US. 2023 Jul 15; 15(13): 6503C6525Read more>>
Mouse liver lysates probed with Galectin 9 Polyclonal Antibody, Unconjugated (bs-1699R) at 1:500 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:10000 for 60 min at 37˚C.
NIH/3T3 lysates probed with Galectin 9 Polyclonal Antibody, Unconjugated (bs-1699R) at 1:500 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:10000 for 60 min at 37˚C.
Molt4 cells were fixed with 4% PFA for 10min at room temperature,permeabilized with 90% ice-cold methanol for 20 min at -20℃, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with Galectin 9 Polyclonal Antibody(bs-1699R)at 1:100 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).