bs-1700R [Primary Antibody]
Wnt3a Polyclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: Wnt3a

Immunogen Range: 281-352/352

Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 89780

Swiss Prot: P56704

Source: KLH conjugated synthetic peptide derived from human Wnt3a

Purification: Purified by Protein A.

Storage Buffer: Aqueous buffered solution containing 1% BSA, 50% glycerol and 0.09% sodium azide.

Storage: Store at -20°C for 12 months..

Background:

Ligand for members of the frizzled family of seven transmembrane receptors. Wnt-3 and Wnt-3a play distinct roles in cell-cell signaling during morphogenesis of the developing neural tube.

Size: 100ul

Concentration: 1ug/ul

Predicted Molecular Weight: 37

Cross Reactive Species: Human
Mouse
Rat

Predicted Cross Reactive Species: Pig

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Gao, Peng, et al. "Salvianolic acid B improves bone marrow-derived mesenchymal stem cell differentiation into alveolar epithelial cells type I via Wnt signaling." Molecular Medicine Reports.Read more>>
  • Lu, Juan, et al. "Expression of Wnt3a, Wnt10b, β-catenin and DKK1 in periodontium during orthodontic tooth movement in rats." Acta Odontologica Scandinavica (2015): 1-7.Read more>>
  • Hayashi, Kouhei, et al. "Involvement of the T-box transcription factor Brachyury in early-stage embryonic mouse salivary gland." Biochemical and Biophysical Research Communications (2016).Read more>>
  • Böscke, Robert, et al. "Wnt Signaling in Chronic Rhinosinusitis with Nasal Polyps." American Journal of Respiratory Cell and Molecular Biology ja (2017).Read more>>
  • Zhu, Afang, et al. "Suppression of Wnt5a, but not Wnts, relieves chronic post-thoracotomy pain via anti-inflammatory modulation in rats." Biochemical and Biophysical Research Communications (2017).Read more>>
  • Jin, Xinhao, et al. "Environmental Enrichment Improves Spatial Learning and Memory in Vascular Dementia Rats with Activation of Wnt/β-Catenin Signal Pathway." Medical science monitor: international medical journal of experimental and clinical research 23 (2017): 207.Read more>>
  • Guo, Furong, et al. "Snail1 is positively correlated with atrial fibrosis in patients with atrial fibrillation and rheumatic heart disease." Experimental and Therapeutic Medicine.(2017)Read more>>
  • Guo et al. Snail1 is positively correlated with atrial fibrosis in patients with atrial fibrillation and rheumatic heart disease. (2017) Exp.Ther.Me. 14:4231-4237Read more>>
  • Sun et al. miR-491-5p, mediated by Foxi1, functions as a tumor suppressor by targeting Wnt3a/β-catenin signaling in the development of gastric cancer. (2017) Cell.Death.Dis. 8:e2714Read more>>
  • Yang et al. MicroRNA-195 acts as a tumor suppressor by directly targeting Wnt3a in HepG2 hepatocellular carcinoma cells. (2014) Mol.Med.Rep. 10:2643-8Read more>>
  • Xu T et al. MicroRNA\708 modulates Hepatic Stellate Cells activation and enhances extracellular matrix accumulation via direct targeting TMEM88. J Cell Mol Med. 2020 Jul;24(13):7127-7140.Read more>>
  • Yang Fan. et al. Catalpol Protects Against Pulmonary Fibrosis Through Inhibiting TGF-1/Smad3 and Wnt/-Catenin Signaling Pathways. Front Pharmacol. 2021 Jan;11:2472Read more>>
VALIDATION IMAGES

L1 rat brain, L2 mouse uterus lysates probed (bs-1700R) at 1:200 in 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 90min in 37˚C. Predicted and observed band size: 37kDa.


Formalin-fixed and paraffin embedded rat ovary tissue labeled with Anti-Wnt3a Polyclonal Antibody (bs-1700R), Unconjugated at 1:200 followed by conjugation to the secondary antibody, (SP-0023), and DAB staining


Lane 1: U2os cell lysates probed with Wnt3a Polyclonal Antibody, Unconjugated (bs-1700R) at 1:300 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.