Host: Rabbit
Target Protein: CD15/Fut4/SSEA-1
Immunogen Range: 251-295/433
Clonality: Polyclonal
Isotype: IgG
Entrez Gene: 14345
Swiss Prot: Q11127
Source: KLH conjugated synthetic peptide derived from mouse CD15
Purification: Purified by Protein A.
Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Background:
Size: 100ul
Concentration: 1ug/ul
Applications:
WB(1:300-5000)
ELISA(1:500-1000)
FCM(1:20-100)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)
Predicted Molecular Weight: 58
Cross Reactive Species:
Human
Mouse
Rat
For research use only. Not intended for diagnostic or therapeutic use.
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- Kim et al. Klotho and S100A8/A9 as Discriminative Markers between Pre-Renal and Intrinsic Acute Kidney Injury. (2016) PLoS.One. 11:e0147255Read more>>
- C Geng et al. A simple fabricated microfluidic chip for urine sample-based bladder cancer detection.(2018).J MICROMECH MICROENG.Read more>>
- Xinchao Miao. et al. Epiprofin Transcriptional Activation Promotes Ameloblast Induction From Mouse Induced Pluripotent Stem Cells via the BMP-Smad Signaling Axis. FRONT BIOENG BIOTECH. 2022 Jun 21;10:890882Read more>>
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Human HL-60 cells probed with CD15 Polyclonal Antibody, Unconjugated (bs-1702R) (green) at 1:100 for 30 minutes followed by a FITC conjugated secondary antibody compared to unstained cells (blue), secondary only (light blue), and isotype control (orange).
Lane 1: MCF-7 lysates probed with CD15/Fut4/SSEA-1 Polyclonal Antibody, Unconjugated (bs-1702R) at 1:300 overnight at 4˚C. Followed by a conjugated secondary antibody at 1:20000 for 60 min at 37˚C.
HL-60 cells were fixed with 4% PFA for 10min at room temperature,permeabilized with 0.1% PBST for 20 min at room temperature, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with CD15/Fut4/SSEA-1 Polyclonal Antibody(bs-1702R)at 1:100 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).