bs-1712R-A594
[Conjugated Primary Antibody]
pan Cytokeratin Polyclonal Antibody, ALEXA FLUOR® 594 Conjugated
DATASHEET
Host: Rabbit
Target Protein: pan Cytokeratin
Clonality: Polyclonal
Isotype: IgG
Source: KLH conjugated synthetic peptide derived from human pan cytokeratin
Purification: Purified by Protein A.
Storage Buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Background:
Cytokeratins are proteins of keratin-containing intermediate filaments found in the intracytoplasmic cytoskeleton of epithelial tissue. The cytokeratins are encoded by a family encompassing 30 genes. Among them, 20 are epithelial genes and the remaining 10 are specific for trichocytes.
In the cytoplasm, the keratin filaments conform a complex network which extends from the surface of the nucleus to the cell membrane. Numerous accessory proteins are involved in the genesis and maintenance of such structure. This association between the plasma membrane and the nuclear surface provides important implications for the organization of the cytoplasm and cellular communication mechanisms. Apart from the relatively static functions provided in terms of supporting the nucleus and providing tensile strength to the cell, the cytokeratin networks undergo rapid phosphate exchanges mediated depolymerization, with important implications in the more dynamic cellular processes such as mitosis and post-mitotic period, cell movement and differentiation. Cytokeratins interact with desmosomes and hemidesmosomes, thus collaborating to cell-cell adhesion and basal cell-underlying connective tissue connection.
Conjugation: ALEXA FLUOR® 594
Excitation/ Emission: 590nm/617nm
Size: 100ul
Concentration: 1ug/ul
Applications:
WB(1:300-5000)
FCM(1:20-100)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)
Predicted Molecular Weight: 42-64
Cross Reactive Species:
Human
Mouse
Rat
Bovine
Cow
Predicted Cross Reactive Species:
Dog
Pig
Horse
Chicken
Rabbit
For research use only. Not intended for diagnostic or therapeutic use.
PRODUCT SPECIFIC PUBLICATIONS
- Chen, Ming, et al. "Periostin activates pathways involved in epithelial-mesenchymal transition in adamantinomatous craniopharyngioma." Journal of the Neurological Sciences (2015).Read more>>
- Cheng, Pang, et al. "Colocalization of Metastasis-associated proteins 1/2 and Estrogen receptor alpha in rat epididymis." Tissue and Cell (2017).Read more>>
- Li, Xin, et al. "Enrichment of Oct3/4‐positive cells from a human bronchial epithelial cell line." Apmis 121.7 (2013): 612-625.Read more>>
- Yang, Wei, et al. "lncRNA H19 is involved in TGF-β1-induced epithelial to mesenchymal transition in bovine epithelial cells through PI3K/AKT Signaling Pathway." PeerJ 5 (2017): e3950.Read more>>
- Wang et al. Anti-proliferative activities of finasteride in benign prostate epithelial cells require stromal fibroblasts and c-Jun gene. (2017) PLoS.One. 12:e0172233Read more>>
- Poruk et al. Circulating Tumor Cells Expressing Markers of Tumor-Initiating Cells Predict Poor Survival and Cancer Recurrence in Patients with Pancreatic Ductal Adenocarcinoma. (2017) Clin.Cancer.Res. 23:2681-2690Read more>>
- echbuhl HM et al. Fibroblast subtypes define a metastatic matrisome in breast cancer. JCI Insight. 2020 Feb 27;5(4). Read more>>
- Mahmoud Labibet al. Tracking the expression of therapeutic protein targets in rare cells by antibody-mediated nanoparticle labelling and magnetic sorting. Nat Biomed Eng. 2020 Jul 27.Read more>>
- Wakiyama H et al. Increased Immunogenicity of a Minimally Immunogenic Tumor after Cancer-Targeting Near Infrared PhotoimmunotherapyCancers (Basel).2020 Dec 12;12(12):3747.Read more>>
- Yasuhiro Maruoka. et al. Interleukin-15 after Near-Infrared Photoimmunotherapy (NIR-PIT) Enhances T Cell Response against Syngeneic Mouse Tumors. Cancers. 2020 Sep;12(9):2575Read more>>
- Yasuhiro Maruoka. et al. Near-Infrared Photoimmunotherapy Combined with CTLA4 Checkpoint Blockade in Syngeneic Mouse Cancer Models. Vaccines-Basel. 2020 Sep;8(3):528Read more>>
- Ryuhei Okada. et al. Near-infrared photoimmunotherapy targeting human-EGFR in a mouse tumor model simulating current and future clinical trials. Ebiomedicine. 2021 May;67:103345Read more>>
- Hui Ren. et al. Arecoline induces epithelial\mesenchymal transformation and promotes metastasis of oral cancer by SAA1 expression. Cancer Sci. 2021 Jun; 112(6): 2173C2184Read more>>
- Verdiana Trappetti. et al. Synchrotron Microbeam Radiotherapy for the treatment of lung carcinoma: a pre-clinical study. Int J Radiat Oncol. 2021 AugRead more>>
- Tomchaney, M.. et al. Paradoxical effects of cigarette smoke and COPD on SARS-CoV-2 infection and disease. Bmc Pulm Med. 2021 Dec;21(1):1-14Read more>>
- Chuanli Zhang. et al. Establishment of a human meibomian gland carcinoma cell model and analysis of differently expressed genes. Exp Eye Res. 2022 Feb;:108983Read more>>
- Yan Wu. et al. A mesenchymal stem cell-derived nanovesicle-biopotentiated bovine serum albumin-bridged gelatin hydrogel for enhanced diabetic wound therapy. MATER DESIGN. 2023 Jun;230:11196Read more>>
- Chen Mingyu. et al. Identification of RAC1 in promoting brain metastasis of lung adenocarcinoma using single-cell transcriptome sequencing. CELL DEATH DIS. 2023 May;14(5):1-14Read more>>
- Denise Bonente. et al. Co-Expression of Podoplanin and CD44 in Proliferative Vitreoretinopathy Epiretinal Membranes. INT J MOL SCI. 2023 Jan;24(11):9728Read more>>
- Fukushima Hiroshi. et al. Intratumoral interleukin-15 improves efficacy of near-infrared photoimmunotherapy. MOL CANCER THER. 2023 JulRead more>>
VALIDATION IMAGES