bs-1712R-A594 [Conjugated Primary Antibody]
pan Cytokeratin Polyclonal Antibody, ALEXA FLUOR® 594 Conjugated
www.biossusa.com
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DATASHEET

Host: Rabbit

Target Protein: pan Cytokeratin

Immunogen Range: 141-240/644

Clonality: Polyclonal

Isotype: IgG

Source: KLH conjugated synthetic peptide derived from human pan cytokeratin

Purification: Purified by Protein A.

Storage Buffer: Aqueous buffered solution containing 1% BSA, 50% glycerol and 0.09% sodium azide.

Storage: Store at 4°C for 12 months.

Background: Cytokeratins are proteins of keratin-containing intermediate filaments found in the intracytoplasmic cytoskeleton of epithelial tissue. The cytokeratins are encoded by a family encompassing 30 genes. Among them, 20 are epithelial genes and the remaining 10 are specific for trichocytes. In the cytoplasm, the keratin filaments conform a complex network which extends from the surface of the nucleus to the cell membrane. Numerous accessory proteins are involved in the genesis and maintenance of such structure. This association between the plasma membrane and the nuclear surface provides important implications for the organization of the cytoplasm and cellular communication mechanisms. Apart from the relatively static functions provided in terms of supporting the nucleus and providing tensile strength to the cell, the cytokeratin networks undergo rapid phosphate exchanges mediated depolymerization, with important implications in the more dynamic cellular processes such as mitosis and post-mitotic period, cell movement and differentiation. Cytokeratins interact with desmosomes and hemidesmosomes, thus collaborating to cell-cell adhesion and basal cell-underlying connective tissue connection.

Conjugation: ALEXA FLUOR® 594

Excitation/ Emission: 590nm/617nm

Size: 100ul

Concentration: 1ug/ul

Applications: FCM(1:20-100)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)

Predicted Molecular Weight: 42-64

Cross Reactive Species: Human
Mouse
Rat
Bovine
Cow

Predicted Cross Reactive Species: Dog
Pig
Horse
Chicken
Rabbit

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Chen, Ming, et al. "Periostin activates pathways involved in epithelial-mesenchymal transition in adamantinomatous craniopharyngioma." Journal of the Neurological Sciences (2015).Read more>>
  • Cheng, Pang, et al. "Colocalization of Metastasis-associated proteins 1/2 and Estrogen receptor alpha in rat epididymis." Tissue and Cell (2017).Read more>>
  • Li, Xin, et al. "Enrichment of Oct3/4‐positive cells from a human bronchial epithelial cell line." Apmis 121.7 (2013): 612-625.Read more>>
  • Yang, Wei, et al. "lncRNA H19 is involved in TGF-β1-induced epithelial to mesenchymal transition in bovine epithelial cells through PI3K/AKT Signaling Pathway." PeerJ 5 (2017): e3950.Read more>>
  • Wang et al. Anti-proliferative activities of finasteride in benign prostate epithelial cells require stromal fibroblasts and c-Jun gene. (2017) PLoS.One. 12:e0172233Read more>>
  • Poruk et al. Circulating Tumor Cells Expressing Markers of Tumor-Initiating Cells Predict Poor Survival and Cancer Recurrence in Patients with Pancreatic Ductal Adenocarcinoma. (2017) Clin.Cancer.Res. 23:2681-2690Read more>>
  • echbuhl HM et al. Fibroblast subtypes define a metastatic matrisome in breast cancer. JCI Insight. 2020 Feb 27;5(4). Read more>>
  • Mahmoud Labibet al. Tracking the expression of therapeutic protein targets in rare cells by antibody-mediated nanoparticle labelling and magnetic sorting. Nat Biomed Eng. 2020 Jul 27.Read more>>
  • Wakiyama H et al. Increased Immunogenicity of a Minimally Immunogenic Tumor after Cancer-Targeting Near Infrared PhotoimmunotherapyCancers (Basel).2020 Dec 12;12(12):3747.Read more>>
  • Yasuhiro Maruoka. et al. Interleukin-15 after Near-Infrared Photoimmunotherapy (NIR-PIT) Enhances T Cell Response against Syngeneic Mouse Tumors. Cancers. 2020 Sep;12(9):2575Read more>>
  • Yasuhiro Maruoka. et al. Near-Infrared Photoimmunotherapy Combined with CTLA4 Checkpoint Blockade in Syngeneic Mouse Cancer Models. Vaccines-Basel. 2020 Sep;8(3):528Read more>>
  • Ryuhei Okada. et al. Near-infrared photoimmunotherapy targeting human-EGFR in a mouse tumor model simulating current and future clinical trials. Ebiomedicine. 2021 May;67:103345Read more>>
  • Hui Ren. et al. Arecoline induces epithelial\mesenchymal transformation and promotes metastasis of oral cancer by SAA1 expression. Cancer Sci. 2021 Jun; 112(6): 2173C2184Read more>>
VALIDATION IMAGES

Hela cells were fixed with 4% PFA for 10min at room temperature,permeabilized with 0.1% PBST for 20 min at room temperature, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with pan Cytokeratin Polyclonal Antibody(bs-1712R)at 1:50 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).