DATASHEET
Host:
Rabbit
Target Protein:
HPV16 E6 + HPV18 E6
Specificity:
This antibody may also detect HPV45 E6, HPV68 E6, HPV39 E6, and HPV35 E6
Clonality:
Polyclonal
Isotype:
IgG
Entrez Gene:
1489078, 1489088
Swiss Prot:
P03126, P06463
Source:
KLH conjugated synthetic peptides from HPV16 E6 + HPV18 E6
Purification:
Purified by Protein A.
Storage Buffer:
0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage:
Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Background:
Human Papilloma virus (HPV) is responsible for the development of type and tissue specific papilloma in the oral cavity and in the larynx. In the skin papilloma virus can cause different types of warts and in the ano-genital region it is associated with condyloma or carcinoma. HPV types 16 and 18 seem to be responsible for the development of some carcinoma like cervical carcinoma.
PRODUCT SPECIFIC PUBLICATIONS
- Yang JH et al. HPV16 E6/E7 upregulate hTERC mRNA and gene amplification levels by relieving the effect of LKB1 on Sp1 phosphorylation in lung cancer cells:. Ther Adv Med Oncol.2020 May 12;12:1758835920917562.Read more>>
- Xiaona Chen. et al. CXCL10 Produced by HPV-Positive Cervical Cancer Cells Stimulates Exosomal PDL1 Expression by Fibroblasts via CXCR3 and JAK-STAT Pathways. Front Oncol. 2021; 11: 62935Read more>>
VALIDATION IMAGES
Antigen: bs-1719P, 0.2ug/100ul \nPrimary: Antiserum, 1:500, 1:1000, 1:2000, 1:4000, 1:8000, 1:16000, 1:32000; \nSecondary: HRP conjugated Goat Anti-Rabbit IgG (bs-0295G-HRP) at 1: 5000; \nTMB staining; Read the data in MicroplateReader by 450nm.
Formalin-fixed and paraffin embedded human colon carcinoma tissue labeled Anti-HPV16 E6 + HPV18 E62 Polyclonal Antibody, Unconjugated (bs-1719R) at 1:200, followed by conjugation to the secondary antibody and DAB staining